内质网应激标志蛋白grp78 在小鼠实验性结肠炎 - 中华临床医师杂志.pdf

·1608 · 中华临床医师杂志( 电子版)2015 年5 月第9 卷第9 期 Chin J Clinicians(Electronic Edition),May 1,2015,Vol.9,No.9 ·基础论著· 内质网应激标志蛋白GRP78 在小鼠实验性 结肠炎中的表达研究 李小珍 金建军 高强 【摘要】 目的 探讨内质网应激标志蛋白 GRP78 在小鼠实验性结肠炎组织中的表达情况。 方法 将8 周龄C57BL/6 小鼠随机分为对照组、葡聚糖硫酸钠（DSS ）组，每组7 只，对照组正常 饮水，DSS 组饮用含2.5% DSS 的饮水，共6 d 建立小鼠急性结肠炎模型。通过疾病活动指数评分 （DAI ）、测量结肠长度、HE 染色病理评分、炎性指标TNFα 和IL-6 mRNA 检测来评估结肠炎症损 伤程度。采用免疫组织化学法和实时定量PCR 法检测结肠组织中GRP78 蛋白和mRNA 表达情况。 结果 对照组未见肠炎表现，DSS 组小鼠出现典型结肠炎表现。DSS 组炎性指标 TNFα 和IL-6 mRNA 均较对照组显著升高。GRP78 蛋白和mRNA 在对照组高表达，在DSS 组表达显著减少（P ＜0.05 ）。 结论 GRP78 在维持肠道正常生理功能中发挥重要作用，炎症状态下其表达受抑制。 【关键词】 结肠炎； 内质网应激； 葡萄糖调节蛋白78； 葡聚糖硫酸钠 Expression of endoplasmic reticulum stress chaperone GRP78 in mouse colitis Li Xiaozhen, Jin Jianjun, Gao Qiang. Department of Gastroenterology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471023, China Corresponding author: Gao Qiang, Email: gaoq37@yeah.net 【Abstract 】 Objective To investigate the expression of ER stress marker protein GRP78 in experimental colitis mice. Methods The 8-week-old C57BL/6 mice were randomly divided into control group and Dextran sulfate sodium (DSS) group. n=7 for each group, mice in control group was given with only drinking water, acute colitis model was established by given with drinking water dissolved with 2.5% DSS to DSS group for 6 days. Disease Activity Index (DAI), colon length and inflammatory scores of colon were observed, inflammatory indicators were measured. The protein and mRNA expressions of GRP78 in colon tissue of mice were evaluated by immunohistochemistry and quantitative real-time PCR respectively. Results There was no colitis in control group, severe colitis were found in