the impact of gsk-3β on the replication of influenza virus in a549 .pdfVIP

Open Journal of Nature Science 自然科学, 2016, 4(4), 371-377 Published Online November 2016 in Hans. /journal/ojns /10.12677/ojns.2016.44045 The Impact of GSK-3βon the Replication of Influenza Virus in A549 Cells 1* 2 2 Xinxian Dai , Lishu Zhang , Hongya Han 1 National Vaccine Serum Institute, Beijing 2 School of Science, Beijing Jiaotong University, Beijing th th st Received: Oct. 12 , 2016; accepted: Oct. 29 , 2016; published: Nov. 1 , 2016 Copyright © 2016 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Open Access Abstract Objective: In order to clarify the role of glycogen synthase kinase-3β (GSK-3β) in the process of in- fluenza virus infection. Methods: We preformed the RNAi to knock down the GSK-3βgene expres- sion, then we determined the virus titers in the A549 cell supernatants by performing TCID 50 as- say, and examined the influence of GSK-3βto cell viabilities of the infected cells as well as the in- fluenza virus-induced A549 cell apoptosis using CCK-8 Kit and Caspase-Glo 3/7 Kit. Results: The results demonstrated that down-regulation of GSK3 expression decreased the viral titers in the A 549 cells supernatants. Down-regulation of GSK-3βexpression decreased the A549 cell viability, at the same time, increased the influenza virus induced cell apoptosis. Conclusion: Hence, GSK-3β negatively regulated the influenza virus-induced host cell apoptosis, and it was essential for the efficient replication of influenza virus. Keywords Virology, Influenza Virus,