金针菇淀粉酶家族基因鉴定及其表达特性分析 - 北京玛格泰克科技发展 .pdfVIP

菌物学报 jwxt@ 22 July 2016, 35(7): 865-873 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q Tel: +86-10 Copyright © 2016 Institute of Microbiology, CAS, all rights reserved. 研究论文 Research paper DOI: 10.13346/j.mycosystema.160060 金针菇淀粉酶家族基因鉴定及其表达特性分析 李肖1 王健1 万芳1 严俊杰1 温志强1 江玉姬2 谢宝贵1* 1 福建农林大学生命科学学院菌物研究中心 福建 福州 350002 2 福建农林大学食品科学学院 福建 福州 350002 摘 要：本研究对金针菇淀粉酶家族基因进行了信息分析，并选用金针菇双核菌株 H1123 作为实验材料，分析了菌丝生 长过程中淀粉酶活性和淀粉酶基因表达特性之间的关系。结果表明，金针菇淀粉酶家族包含6 个α淀粉酶和 1 个γ 淀粉 酶。7 个淀粉酶基因的表达量均在菌丝接种后第 10 天出现峰值，并与胞外淀粉酶活性呈同步变化，说明基质中淀粉的分 解和利用是淀粉酶家族各成员之间相互协调的结果。其中 α-Amy-1 、α-Amy-4 、α-Amy-5 的上调幅度最大，为淀粉降解和 代谢过程的主效基因。值得注意的是胞内淀粉酶基因 α-Amy-1 在第 10 天时达到约90 倍的上调表达水平。我们推测：金 针菇胞外淀粉酶将淀粉分解为小分子单糖的同时，其胞内淀粉酶也参与了这些糖类的吸收和运输过程。 关键词：金针菇，淀粉酶，酶活，基因表达，相关分析 Amylase family gene identification and the expression profiling of the genes in Flammulina velutipes 1 1 1 1 1 2 1* LI Xiao WANG Jian WAN Fang YAN Jun-Jie WEN Zhi-Qiang JIANG Yu-Ji XIE Bao-Gui 1 Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China 2 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China Abstract : The amylase family genes in the genome of Flammulina velutipes were identified and analyzed. The correlation between the expression levels of amylase family genes and the activity of amylase were analyzed in the F. velutipes dikaryotic strain H1123. It was turned out that there were six α-amylases and one γ-amylase in F. velutipes. All the amylase genes showed the highest expression levels on the 10th day after mycelium incubation, being consistently correlated with the activity of extracellu