不同级别胶质瘤细胞系重叠基因的鉴定 - 第三军医大学学报.docVIP

不同级别胶质瘤细胞系重叠基因的鉴定 童建国，曾义2，杨忠3*，游潮2 1. 重庆市肿瘤研究所神经外科， 重庆 400030 2. 四川大学华西医院神经外科，四川 成都 610041 3. 第三军医大学神经生物学教研室，重庆400038 通讯作者：杨忠，第三军医大学神经生物学教研室，重庆400038 Identification of the overlapped genes in different grades glioma cell lines TONG Jian-guo 1, ZENG Yi 2, YANG Zhong 3*, YOU Chao 2 1. Department of Neurosurgery, Chongqing Tumor Hospital, Chongqing 400030 2. Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041 3. Department of Neurobiology, Third Military Medical University, Chongqing 400038 【摘要】 目的：利用cDNA微阵列分析不同级别和全反式维甲酸诱导的胶质瘤细胞系间的重叠基因。方法：通过运用cDNA微阵列技术比较两种不同级别胶质瘤细胞系CHG-5（WHO Ⅱ级）和SHG-44（WHO Ⅳ级）以及10 μmol/L全反式维甲酸诱导3天的SHG-44细胞系的基因表达谱，鉴定与胶质瘤进展相关的重叠基因，并消除细胞系间的个体差异。随机选择数个基因进行Northern杂交实验，以验证cDNA微阵列结果的。按功能分为：凋亡类、细胞转移类、细胞周期与生长调节类、细胞骨架类、细胞分化类、细胞代谢类、癌基因、氧化磷酸化类、受体与信号传导类、核蛋白体类、泛素-蛋白酶系统类、生长因子与细胞因子类及其他基因Northern杂交实验验证Abstract] OBJECTIVE: To identify the overlapped genes from different grades and all-trans retinoic acid treated glioma cell lines by cDNA microarray. METHODS: By comparing the gene expression profiling between two different grades glioma cell lines (CHG-5, WHO grade Ⅱ; SHG-44, WHO grade Ⅳ) and all-trans retinoic acid (ATRA) treated SHG-44 for 3 days by using a cDNA microarray, a panel of overlapped genes that related to glioma progression was obtained, and the cell line individual variation was avoided as well. To confirm the results of genes detected by the cDNA microarray, some differentially expressed overlapped genes were selected randomly for Northern blot analysis. RESULTS: Thirty-one overlapped genes were identified by cDNA microarray in this study, among them, 20 were up-regulated and 11 were down-regulated. These genes were functionally classified into several groups as follow: apoptosis, cell mobility and metastasis, cell cycle and growth regulation, cytoskeleton, differentiation, metabolic pathway, oncogene, oxidative phosphorylation, receptors and signal transduction, ribosome, ubiquitin-proteasome system, growth factor and cyt