同型半胱氨酸对人脐静脉内皮细胞线粒体膜电位的影响 - new century .pdfVIP

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同型半胱氨酸对人脐静脉内皮细胞线粒体膜电位的影响 - new century .pdf

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同型半胱氨酸对人脐静脉内皮细胞线粒体膜电位的影响 - new century

/index.php/gjccd Global Journal of Cardiovascular and Cerebrovascular Diseases，2013,Vol.1,No.1 Effect of homocysteine on mitochondrial membrane potential of human umbilical vein endothelial cells 1 1* 1 2 1 SHI Yun-qi , WANG Hao ,LI Zhan-quan ,ZHANG Jing-sheng ,HU Wen-xu 1 Liaoning Provincial Peoples Hospital Cardiology,Shenyang 110016,China 2.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China Received: Nov 10, 2013 Accepted: Nov 27, 2013 Published: Dec 22, 2013 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Objective: To observe the changes of endothelial mitochondrial membrane potential （MMP ）by building Hcy-induced endothelial impairment model in cultured human umbilical vein endothelial cells and to analyze the mechanism of Hcy-induced apoptosis.Methods:(1) Cultured HUVECs of term infant in vitro provide enough experimental materials. (2)The HUVECs are randomly divided into five groups: the control group does not add Hcy, the other groups add 0.01、0.1、1.0、3.0mmol/LHCY respectively, and put them together for 24 hours to detect the changes of MMP of the HUVECs at 3 、6、12、24 hour.Results:There is no difference in the level of MMP in the HUVECs stimulated by HCY for 24 hours between the HCY0.01mmol/L group and the control group. Comparing with the control group,MMP reduces at 24 hour in the HCY0.1 mmol/L group ,at 6、12、24 hour in the HCY1.0 mmol/L group and at 3、6 hour(P0.05 ）and obviously at 12、24 hour(P0.01) in the HCY3.0 mmol/L group.In the same Hcy-induced group, MMP obviously reduces at 24 hour, ＞12h、6h、3h.At the sa