姜黄素对阿尔茨海默病模型大鼠海马gsk3β蛋白表达的影响 叶茂盛周 .docVIP

姜黄素对模型GSK3β蛋白的 叶茂盛 周勇 蒋蓝英 (浙江中医药大学附属中西医结合，，310003) [摘要] 目的 探讨(AD)模型大鼠能力β（GSK3β）蛋白表达的影响。方法 将60只SD雄性大鼠4组：假手术组A组）、Aβ模型组(B组)和干预组(C组)(D组)。B组直接注射Aβ1-42于大鼠海马A1区；C组用浓度30 d。测试大鼠学习记忆能力，PCR逆转录聚合酶链反应检测GSK3β表达水平结AD模型大鼠水迷宫测试的潜伏大鼠学习记忆能力。Aβ模型组GSK3β蛋白表达明显增强,其阳性单位面积和平均吸收光密度值显著高于假手术组(P＜0.01)；姜黄素干预GSK3β蛋白表达明显减弱,其阳性面积单位和平均吸收光密度值低于模型组(P＜0.01)。结论 可明显改善AβAD大鼠学习记忆功能可显著大鼠海马GSK3β蛋白改善Aβ的[关键词] 阿尔茨海默病；；β；细胞凋亡 Effect of curcumin on GSK3β expression in the hippocampus of Alzheimer′s disease model rats YE Maosheng, ZHOU Yong,JIANG Lanying,ZHAO Dongjie,LI Danming (Department of Gerontology, Affiliated to Integrated Traditional Chinese and Western Medicine Hospital of Zhengjiang Chinese Medical University,Hangzhou 310003,China) [Abstract] Objective To investigate the effect of curcumin on the ability of learning and memory, the expression of glycogen synthesis kinase 3β（GSK3β) in the hippocampus area of Alzheimer′s disease model rats. Methods Sixty Sprague-Dawley rats were assigned into 4 groups. Rats in the sham-operated group(A group) were injected with normal saline. Rats in the Aβ group(B group) , low-dose curcumin group (C group) and high -dose curcumin group (D group) were injected with Aβ1-42. Rats in the curcumin group (C group and D group )were feeded with curcumin solution with different dose for 30 days consecutively. The Morris water maze was used to investigate the ability of learning and memory in the rats.The GSK3βprotein expression were detected by PCR reverse transcription polymerase chain reaction.Results Curcumin can shorten the incubation period of experimental rats testing in water maze.Compared to sham-operated group,the exprssion of GSK3β positive area,average optical density in hippocampus area CA1 of group Aβ model group increased significantly(P0.01).The exprssion of GSK3β positive area,average optical density of curcumin group were decreased than that in Aβ group, the difference between the two groups is statistically significant (P0.01).Conclusion Curcumin can amelior