乙醛在小鼠酒精依赖性行为中的作用研究 - 生命科学研究 - 湖南师范大学.doc

乙醛在小鼠酒依赖性行为中的作用研究 宁玲玲，戴长亮，谭嵘，彭逊，薛媛，陈湘定( （ 湖南师范大学 生命科学学院，中国湖南 长沙 410081） 摘 要：乙醛为酒精代谢的中间产物，但其在酒依赖中的作用不清楚.通过条件化位置偏好（CPP）和条件化味觉偏好（CTP）试验，分析乙醛对小鼠乙醇依赖性行为的影响，研究乙醛在酒依赖中的作用.研究发现，经0.8%乙醇预处理7 d后，小鼠训练8次则表现出对乙醇的条件化位置偏好(n=6,P0.01)，而经乙醛训练的小鼠则对乙醛无明显条件化偏好行为(n=6,P0.05).当用0.8%乙醇、0.4%乙醛混合训练乙醇依赖性小鼠时，其位置偏好行为减弱(n=6,P0.01).10%乙醇预处理的小鼠味觉偏好乙醇(n=6,P0.01)，而当乙醇中加入1%乙醛时，其味觉偏好现象减弱(n=6,P0.01).1%乙醛训练7 d后的小鼠不表现对乙醇的味觉偏好，但选择摄入乙醛及乙醇、乙醛混合溶液的量有所增加.结果表明乙醛在小鼠酒依赖行为中可能存在一定促进作用. 关键词：乙醇；乙醛；酒依赖；条件化位置偏好；条件化味觉偏好 中图分类号： Q955 文献标识码：A 文章编号：1007-7847（2009）00-0000-00 Research on the Effects of Acetaldehyde on Alcohol Dependence Behavior in Mice NING Ling-ling, DAI Chang-liang,TAN Rong, PENG Xun，XUE Yuan, CHEN Xiang-ding* (College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China) Abstract: Acetaldehyde is an intermediate of alcohol metabolism, but its effect on alcohol dependence is not clear. It was performed with conditioned place preference (CPP) and conditioned taste preference (CTP) experiments to investigate the effects of acetaldehyde on the alcohol dependence in mice. The mice showed significant CPP to ethanol (n=6, P0.01) while training with ethanol for 8 times after pre-treatment with 0.8% ethanol for 7 days, but no significant CPP effect on acetaldehyde was observed (n=6, P0.05). While trained with 0.8% ethanol and with 0.4% acetaldehyde, the CPP effect to ethanol decreased (n=6, P0.01). Mice also showed the CTP to ethanol after drinking water and 10% ethanol for 7 days (n=6, P0.01), but the CTP to ethanol decreased while 1% acetaldehyde was added in drinking water. Mice showed no CTP to ethanol after treated with 1% acetaldehyde for 7 days, but the drinking to the admixture of ethanol and acetaldehyde increased. The results showed that acetaldehyde had important roles in alcohol dependence behavior in mice. Key words: ethanol；acetaldehyde； alcohol dependence； conditioned place preference； conditioned taste preference （Life Scien