槟榔果实酚类化合物抗氧化力及其添加于口含薄片之探讨.docVIP

檳榔果實酚類化合物抗氧化力及其添加於口含薄片之探討 Studies on the antioxidant activity of polyphenols in areca fruit and their application in troche film 研究生：佘雅琳 She, Ya-Lin 指導教授：蔡碧仁 Tsai, Pi-jen 劉展冏 Liu, Chan-Chiung 【摘要】 檳榔果實 (Areca catechu Linn.)中的主要成分包含多酚化合物、生物鹼、粗纖維、脂肪、多醣類等，其中多酚化合物具有抗氧化、抗致突變性等性質，為一種天然的抗氧化物，且台灣檳榔果實產量豐富，常有大量廢棄檳榔果實的產生，若能加以利用，可增加檳榔果實之利用價值，故本研究以檳榔果實為原料，先行探討其最適萃取條件，然後取不同品種 (白肉、紅肉、白尖)、季節 (夏天、冬天)及粒徑大小 (size I、II、II)的檳榔果實探討其酚類化合物及抗氧化力，並進ㄧ步了解其中抗氧化力貢獻來源物質，測定項目包括總酚、原花青素含量，抗氧化力 (FRAP還原力及DPPH自由基清除能力)，膠體層析以及HPLC、ESI-MS鑑定；另外，爲增加檳榔果實之應用性，萃取其酚類化合物添加於口含薄片中，結果顯示，選用酒精為萃取溶，在70%的酒精及果實與溶液比例1:10AP還原力、DPPH自由基清除能力size III為最高，size II次之，size I為最低。相關性統計分析顯示，總酚與原花青素之含量呈顯著相關，此二者又分別與FRAP還原力及DPPH自由清除能力呈高度相關FRAP還原力及DPPH自由清除能力95% alcohol及50% acetone去沖提時，不論品種、季節粒徑來說，95alcohol及50% acetone二者皆可各沖提出兩個區分物，將此四個區分物分別測定其抗氧化力發現，50% acetone萃取的第二區分物 (FII) 具有最高抗氧化力，95% alcohol FII次之。經HPLC進一步分析證實，95% alcohol FII包含catechin及epicatechinESI-MS檢測，發現50% acetone FII包含有二聚體及三聚體之原花青素，故推測檳榔果實抗氧化力與原花青素有關AP還原力、DPPH自由基清除能力粒徑大小Areca fruits (Areca catechu Linn.) are rich in polyphenols, alkaloids, natural fiber, lipid, and polysaccharides. Polyphenols are crude antioxidant which exhibit antioxidant activity, antimutagenicity, etc. Areca fruits are one of the abundant produces in Taiwan and a large quantity wastes are generated annually. We can make good use of areca fruits to improve economical value of areca fruits. In this study, optimum extraction method for extracting polyphenols from areca fruits were investigated. The aim of this study is to elucidate the effect of different varieties (white, red and white sharp), harvest seasons (summer and winter) and size (size I, II and III) on the polyphenols and proanthocyanidin contents of areca fruits, and try to understand the contribution of these components on antioxidant activity. Analyses include antioxidant component (total polyphenols and proanthocyanidins concentration), antioxidant capacity (FRAP and DPPH scavenging ability), gel filtration, HPLC analysis and ESI-MS a