菌种保存方法（Bacteria preservation method）.docVIP

菌种保存方法（Bacteria preservation method） Characteristics of microorganisms with easy variation and therefore in the preservation process, must make the microbial metabolism in the inactive or relatively static state to within a certain period of time the variation and keep life ability does not occur. Low temperature, drying and isolating air are important factors to reduce microbial metabolic capacity. Therefore, there are many methods to preserve bacteria, but they are designed according to these three factors. Preservation methods can be broadly divided into the following: 1. passage culture method There are slant culture, puncture culture, blister meat culture, etc. (the latter is used for preservation of anaerobic bacteria). After culture, it is kept in the refrigerator at 4 - 6 DEG C. 2. liquid paraffin covered method Is a method of subculturing, can prolong the preservation time, it is the culture and puncture in the slant culture on liquid paraffin covered sterilization, one can prevent medium moisture evaporation caused by bacterial death, on the other hand can prevent the entry of oxygen to reduce metabolism. 3. carrier preservation It is a method of preserving microorganisms on appropriate carriers such as soil, sand, silica gel, filter paper, and then for drying. For example, sand preservation and filter paper preservation, they are widely used. 4. host conservation For it is not in the artificial medium on the growth of microorganisms, such as viruses, Rickettsia multocidatreponema, they must be in the life of the animal, insect, chicken embryo infection and passage, which is equivalent to the general microbial culture preservation. Viruses and other microorganisms can also be preserved by other methods, such as liquid nitrogen storage and freeze drying. 5. cryopreservation Can be divided into low temperature refrigerators (-20 - -30, -50 - -80 DEG C), dry ice, alcohol, rapid freezing (about -70 degrees Celsius) and liquid nitrogen (-196 degrees Celsius) an