A plasmonic antenna-in-box platform for enhanced single-molecule analysis at micromolar concentrations.（用于在微摩尔浓度下增强单分子分析的等离子体天线盒平台。）.pdfVIP

LETTERS PUBLISHED ONLINE: 9 JUNE 2013 | DOI: 10.1038/NNANO.2013.98 A plasmonic ‘antenna-in-box’ platform for enhanced single-molecule analysis at micromolar concentrations 1 2 1 2 ´ 1 Deep Punj , Mathieu Mivelle , Satish Babu Moparthi , Thomas S. van Zanten , Herve Rigneault , 2,3 ´ ´ ´2,3 ´ ˆ 1 Niek F. van Hulst , Marıa F. Garcıa-Parajo and Jerome Wenger * Single-molecule fluorescence techniques1–3 are key for a the fabrication of thousands of such antennas can be envisioned on number of applications, including DNA sequencing4,5, mole- a microscope coverslip. 6,7 8 cular and cell biology and early diagnosis . Unfortunately, As well as the local enhancement and screening arising from observation of single molecules by diffraction-limited optics the antenna-in-box, molecular photodynamics can be exploited to is restricted to detection volumes in the femtolitre range further improve the contrast between the hot spot enhanced emis- and requires pico- or nanomolar concentrations, far below sion and the background fluorescence. Fluorescent emitters with 2 the micromolar range where most biological reactions occur . low quantum yield exhibit much higher enhancement factors than This limitation can be overcome using plasmonic nano- emitters with high quantum yield, because the low quantum yield structures, which enable the confinement of light down to allows a larger benefit to