共轭亚油酸对甲醛致血管内皮细胞损伤的保护作用.pdfVIP

实用预防医学 2008年6月 第l5卷 第3期 Practical Preventive Medicine，Jun．2008，Vol 15，No．3 645 文章编号：1006—3110(2008)03—0645—03 【论 著 】 共轭亚油酸对甲醛致血管内皮细胞损伤的保护作用 贺栋梁 ，李梓民。，英 姿 ，吴成秋 摘要： 目的 观察共轭亚油酸(CLA)对甲醛致内皮细胞株(HUVEC一12)损伤的影响及其作用机制。 方法 HUVEC一12体外培养至对数生长期，分别用浓度为0．0、50．0、100．0,200．0／~mol／L的CLA预孵12 h，再经0．1 mmol／L 甲醛处理，电镜观察细胞形态学变化，分光光度法检测细胞培养液中LDH、MDA和NO的含量变化。 结果 甲醛可致 内皮细胞损伤，导致细胞膜破裂，甲醛损伤组(0．0／~m0t／LCLA)细胞LDH、MDA和NO释放量高于正常对照组，差异有显 著性(P0．01)；与甲醛损伤组比较，5o．0 mol／L CLA组内皮细胞 LDH释放量低(P0．01)，100．0及200．0／~mol／L CLA 组LDH、MDA和NO释放量均下降(P0．01) ，但100．0及200．0／~mol／L CLA组间差异无显著性。 结论 甲醛致内皮 细胞损伤与细胞脂质过氧化有关，适当浓度的cLA可减轻这种损伤。 ’ 关键词：共轭亚油酸；甲醛；血管内皮细胞；脂质过氧化 中图分类号：R541．4 文献标识码：A Protective Role of Conjugated Linoleic Acid Against Damages of HUVEC一12 Induced by Formaldehyde HE Dong—liang ， LI zz—ruing ，I11 Zi ，et a1．(1．School ofPublic Health，University ofSouth China，Hengyang 421001，Hunan，China； 2．Hunan Provincial Center for Disease Control and Prevention，Changsha 410005，Hunan，China) Abstract： Objective To study the injury and its mechanism of formaldehyde on HEVEC一12 and the protective effects of conjugated linoleic acid(CLA)． Methods HUVEC一12 was cultured with DMEM in vitro and the cells were selected in the exponential phase of growth for experiment．The cells were pretreated with 0．0，50．0，100．0，and 200．0 moI／L CLA and cultured with 0．1mmol／L formaldehyde．The cell morphology was monitored by transmission electron microscope(TEM)．The cOncentratiOns of MDA，transudation contents of LDH and NO were measured by spectrOphOtOgraphy to evaluate the oxidative damage and possible mechanism． Results Formaldehyde induced cell membrane injury：The levels of LDH and MDA in 0．1 mmol／L formaldehyde group were significantly increased as COWIpared with th