植物实时荧光定量PCR内参基因的选择_胡瑞波.pdfVIP

, 2009, 11( 6) : 30 - 36 Journa l o f Ag ricu ltura l Sc ience and T echno logy PCR 胡瑞波, 范成明, 傅永福 ( , , 1000 81) : RT-PCR ( rea l-tmi e qu antitative reverse transcription PCR, qRT-PCR ) , qRT-PCR , , qRT-PCR E ST qRT-PCR, qRT-PCR , : RT-PCR geN o rm : Q 789 : A : 1008-0864 ( 2009) 0 6-0030-07 ReferenceGene Selection in PlantRea-l timeQuantitative Reverse Transcription PCR (qRT-PCR) HU Ru -i bo, FAN Cheng-m ing, FU Y ong-fu ( Inst itu te of C rop Science, N at ion al K ey F aci lity of C rop G en e R esou rce and G enetic Im provem en t, Ch in ese A cadem y of A gricultura l S cien ces, B eijing 100081, Ch ina) Abstract: R eal- tmi e quantitativ e RT-PCR ( qRT-PCR ) techno logy, w ith qu antitative accuracy, h igh sen s itiv ity and high-throughput ch aracter istics, h as been w ide ly u sed in gene expression analysis. Based on the re la tive qu an tita tive ana lys is, qRT-PCR data mu st be norm a lized w ith one o r mo re prop er and stab le in ternal reference genes. H ou se- keep ing genes are cu stom ar ily u sed as endogenou s references for re lative qu an tif ication. But no t a sing le g ene can act as a un iv ersal reference reported so far. M ost o f the trad itiona l hou sekeep ing genes a re unab le to en sure accurate norm a lization in qRT-PCR. Based on the trem endou s gene-chip expression da ta and public deposited EST data, new reference genes w ith sup erior stab ility w ere selected and ver if ied w ith qRT-PCR. T he research progress of referen