脉冲电磁场对家猪淋巴细胞的细胞遗传学效应.pdfVIP

维普资讯 动 物 学 研 究 2001，Apr．22c2)：89—92 CN53—1040／Q ISSN 0254—5853 ZoologicalResearch CytogeneticEffectsofPulsingElectromagneticFieldonDomesticPig LymphocytesinVitro ZOU Fang··Dong XU Liu WANGZi--Shu WANGXi--Zhong (Col~geof ，Sich~n ¨吖，Chengdu 610064．China @腓 educ) Abstract：Theeffectsofpulsingelectromagneticfields(PEMFs)oncells~Rtevery．impo~antsubjectsinthefieldof bioelcetromagnefics．h thisexperiment．htecytogeneticeffectsofPEMFondomesticpiglymphocytes retested ／nv／t· fo．Piglymphoc)~esinRPMI1640medium woreexposedtoPEMFsof100kHz∞d200kHzfor12．24and48hours． Chromosomalaberrahons(aneuploidy，breaks，gaps．ete1) fignlficandyincreasedinexposedcultures．andof theseaberrations，56％ chromosomalorchromatidbreaksand42％ gapsinducedbyPEMFswerehtepointsofpigchro· mosomalfragilesites．Thebowlinefrequencyofsister-chromatidexchange(SCE)increasedafterexposinglymphoeytes continuouslytoPEMFsof100kHzand200kHzfor48hours TheseresultssuggestedthattheexposuretoPEMFsmiShtin· dueeatypeofDNA lesionnadchromosomal aberrations． Keywords：Pulsingelcetromagneticfields；Peripheralbloodlymphocyte；Chromosonudaberrations；Fragilesites Th pulsingelectromagneticfields(PEMFs)re· beenshownforhumanlymphocytes (Coheneta1．， sultingfrom lighthingstrokes，nuclearburstsandradar 1986)．NegativeresultsregardinghtelevelofSCE transmittersaswellasfrom high-vohageelectricalener· have also been reportde in Chinese hams~r cells gYgeneration are fairly wellknown．Electromagnetic (Takabashieta1．，1987)andinbovine(Ambresioet fieldshavebeenshowntoinfluenceavarietyofbiologi· a1．， 1985) nad human lympheeytes (Khalil ＆ catprocesses，includingcellproliferation(Co