树突状细胞分布与糖尿病大鼠主动脉内膜增生的关系.pdfVIP

· 196· 南方医科大学学报fJ South Med Univ) 2008；28(2) 树突状细胞分布与糖尿病大鼠主动脉内膜增生的关系 傅 强，李志梁，傅小华，雷 霄，缪 绯，吴宏超，刘映峰(南方医科大学珠江医院心内科，广东 广州 510282) 摘要：目的探讨糖尿病大鼠树突状细胞在外周血、动脉壁以及脾脏分布情况及与主动脉内膜增生的关系。方法链脲 菌素腹腔注射及经高脂饮食喂养8周形成糖尿病大鼠内膜增生模型，分别取外周血、动脉壁以及脾脏组织，并制备组织 单细胞悬液。应用流式细胞仪检测树突状细胞及T淋巴细胞比例情况。结果糖尿病大鼠及高脂饮食喂养合并糖尿病 大鼠内膜较正常对照大鼠内膜增生明显(P0．05)，其外周血树突状细胞分布明显减少且主动脉内树突状细胞比例明显 升高(P0．05)。T淋巴细胞比例检测同样发现，糖尿病大鼠及高脂饮食喂养合并糖尿病大鼠较正常大鼠，外周血及主动 脉内T淋巴细胞比例升高(P0．05)。结论糖尿病大鼠及高脂饮食喂养的糖尿病大鼠主动脉内膜增生与树突状细胞及 T淋巴细胞比例变化呈一定相关关系，树突状细胞等免疫细胞在糖尿病大鼠主动脉内膜增生形成中可能起重要作用。 关键词：树突状细胞；糖尿病；动脉粥样硬化；主动脉；内膜增生 中图分类号：R392．12 文献标识码：A 文章编号：1673-4254(2008)02．0196．04 Association of dendritic cell distribution with intima hyperplasia in rats with diabetes mellitus FU Qiang，LI Zhi-liang，FU Xiao-hua，LEI Xiao，MIAO Fei，、 1L，Hong-chao，LILT Ying-feng Department ofCardiology，Zhujiang Hospital，Southern Medical University，Guangzhou 5 10282，China Abstract：Objective To investigate the association of dendritic cell distribution in the peripheral blood，spleen and arterial wall with inthnal hyperplasia in rats with diabetes mellitus．Metllods Diabetes mellitus was induced in rats by intraperitoneal injection of streptozotocin and high-fat feeding for 8 weeks．Peripheral blood,arterial wall and the spleen were collected五-0m the rats to prepare cell suspensions，in which the proportions ofdendritic ceils and T cells were determined by flow cytometry． Results The tunica intimal hyperplasia was more obvious in diabetic rats with or without high-fat feeding as compared、 m that ofthe control rats(尸l0．05)，and their dendritic cells decreased significantly in the peripheral blood(尸l0．05)but increased inthe arterialwal1．Th epercentageofT cellsWas alsoincreasedintheperipheralbloodand arterialwallofthediabeticrats． Conclusion Changes in the distribution ofdendritic ceils and T cells are closely associated m intimal hyperplasia in diabe