茶油抑菌效果的研究.pdfVIP

安徽农业科学，Journal of Anhui A ．Sei．2008，36(14)：5913—5914 责任编辑 郑丹丹 责任校对 况玲玲 茶油抑菌效果的研究 蒋玲艳，王林果，欧熳熳 (玉林师范学院化学与生物系，广西玉林537000) 摘要 f目的]研究茶油的抑茵性能及其对常见微生物的抑制效果。f方法1采用滤纸片法研究荼油的抑茵能力；用气相色谱法对茶油 中的脂肪酸成分进行简单的测定。『结果]茶油对细菌、霉菌和酵母均具有很好的抑菌作用，茶油对金黄色葡萄球菌、大肠杆菌、枯草 芽孢杆菌、黑曲霉和米曲霉、啤酒酵母的最低抑茵浓度分别是3％、4％、7％、3％、1％，其抑茵效果与100 txg／ml的氨苄青霉素效果相 当。茶油在DH值4～9的范围内具有明显的抑菌作用。茶油溶液经80、100、121℃加热处理20min后，荼油仍保持艮好的抑菌效果。 『结论]茶油对常见的微生物具有很好的抑制效果，且其抑茵能力几乎不受pH值影响，热稳定性高。 关键词 茶油；气相色谱；抑菌效果；热稳定性 中图分类号 $571．1 文献标识码 A 文章编号 0517—6611(2008)14—05913—02 Study on the AntimicrobiM Effect of the Camellia OiI JIANG Ling-yan et al (Department of Chemistry and Biology，Yulin Normal Universky，Yulin,Guangxi 537000) Abstract 『0biective]The study aim to research the antimicrobial eapability of camellia oil and its antimicrobial effect of camellia oil on the COlillilOn microbe．[Method]The filter paper method Was used to study the antimicrobial capability of camellia Oil and the gas chromatography method Was used to measure the fatty acid composition Of camellia oil．『Result]The Camellia oil coutd effectively inhibit the experimental microorganism such as bacteria mildew and yeast．The minimal inhibitory concn．of camellia oil to Taphylococcus aureaus，Escherichia coli， Bacillus subtilb Asper~illus n r and A spergillus oryzae and Saccharomyes cerevisiae were 3％，4％，7％，3％and 1％，resp．The antimicrobial effect was equal to that of ampinicinin at 100 Ixg／m1．The camellia oil had obvious antimicrobial effect when pH was in 4N9 and after its solution Was treated by 80．100 and 121oC for 20 minutes：[Conclusion]The camellia oil could inhibit the familiar microorganism better and its antimierobial function was hardly influenced by pH and heating．SO it had higher thermosta】)ilifv． Key words Camellia oil；Gas chromatography；Antimicrobial effect；Heat staj)il 茶油，又称茶籽油、山茶油，是由茶树的果实——油茶 茶油浓度的增加菌体数减少，到一定浓度时无菌生长，该浓 籽经过加工、提炼而得到的一种食用油。茶油作为我国最古