柴胡止血液对人工性周期大鼠置器后子宫内膜NO和NOS动态变化的影响.pdfVIP

2008年第26卷第3期 四 川 中 医 Vo1．26，No．3，2008 Journa1 of Sichuan of Traditional Chinese Medicine · 17 · 柴 胡 止 血 液 对 人 工 ，I生周 期 大 鼠 置 器 后 子 宫 内 膜 NO和 NOS动 态 变 化 的影 响 米 周 宜 陈 钢 沈映君 成都中医药大学 (四川成都 610075) 摘要：目的：研究柴胡止血液影响安放IUD后大鼠性周期内子宫组织NO、NOS动态变化的规律，进一步明确NO 在 IUD出血副反应中的作用，为临床治疗提供新的思路和方法。方法：建立人工性周期的动物模型，并用阴道涂片的 方法测定其阴道上皮细胞角化率，观测安放 Cu—IUD大鼠在人工性周期的不同时相，子宫组织 NO含量及 NOS活性的 动态变化以及柴胡止血液对其产生影响。结果：安放IUD后，整个周期 NO的含量均增高，其变化规律和iNOS(刺激 型NOS)的变化规律较为一致，柴胡止血液能通过降低 iNOS的活性来降低 NO的分泌。结论：安放IUD后，对大鼠子 宫组织N0、NOS的动态变化产生了影响，而柴胡止血液能对抗安放 IUD所造成的影响。 关键词：宫内节育器 一氧化氮 一氧化氮合酶 中图分类号：R285．5 文献标识码：A 文章编号：1000—3649(2008)03一oo17 O3 Effect of Ch棚 uZlliXueYe on Dynamic Regular Patterns of NO and NOS in 1UD Located artificial sexual cycle Rat Model／ Zhou Yi∥Chengdu University ofTCM (Chengdu Shuan 610075。China) Abstract Objective：To study the effect of chaihuzhixueye on dynamie regular patterns of NO and NOS in artificial sexual cycle rat model whose uterus was located with intrauterine device(IUD)-in order to explain the influence of N0 in IUD induced hemorrhage and therefore provide new method in clinic treatment．Methods：Artificial sexual cycle rat model was established-keratinocyte ratio of vagina epithelia was determined by the method of vagina smear．In the different period of artifieial sexual cycle，NO content and the dynamie regular pattern of NOS in uterine tissue was determined on IUD located rat．also the effect of ehaihuzhixueye was studied．Re— sults：The NO content was increased during the whole art班cial sexual Cycle after IUD was located．and the variation of NOS was similar with iNOS．The secretion of N0 could be decreased by ChaIhuzhixueve through reducing the activity of NOS．Conclusion：Chaihuzhixu— eye could inhibit the change of dynamic regular pat