绵羊QM基因的电子克隆及其生物信息学分析.pdfVIP

安徽农业科学．Journal of Anhui Agn．Sci．2008，36(14)：5767—5769，5898 责任编辑 王森 责任校对 卢瑶 绵羊aM基因的电子克隆及其生物信息学分析 尹珊珊，吴登俊，张翔宇，周明亮 (四川农业大学动物科学与技术学院，四川雅安625014) 摘要 『目的]为研究绵羊QM基因的生物学功能奠定基础。【方法]利用EST数据库和电子克隆等技术克隆绵羊QM基因，通过生物信 息学分析预测其序列结构，检测其在绵羊不同组织中的表达情况并分析其与其他14物种QM基因的同源性。【结果]首次成功克隆了 绵羊QM基因，全长771 bp，含有1个最长为645 bpORF，编码的蛋白分子量为24．61 kD，为碱性蛋白质。QM蛋白的二级结构含有6 个 一螺旋和1O个B一折叠，其余为无规卷曲。QM基因在绵羊脾脏、脑组织和骨组织中较好表达，在其余7种组织中也均有表达。QM 基因在病变组织中表达量比正常组织高。在14个物种中绵羊QM基因与牛的进化距离最短。【结论]QM基因与肿瘤抑制、细胞生长、 分化、发育和凋亡等有关，且有极高保守性。 关键词 绵羊；QM基因；电子克隆；表达序列标签 中图分类号 $826 文献标识码 A 文章编号 0517—6611(2008)14—05767—03 Analysis of Silico Cloning of QM Gene in Sheep and Its Bioinformatics YINShan~han etal(Co11ege ofAnimal Science andTechnology，SichuanAgriculturalUniversity，Ya an，Sichuan 625014) Abstract【Objective]The research aimed to 1ay the foundation for studying the biological functions of QM gene in sheep_【Method]QM gene in sheeD was cloned by using EST database，in silico cloning，etc．and its structure sequence was predicted through bioinformatics analysis．Its expression in different tissue of sheep was detected and its homology with 14 other species were compared．【Result]QM gene in sheep was cloned for the 1st time with total length of 771 bp，containing an ORF with the 1ongest length of 645 bp．Its molecular weight of the coded protein was 24．61 kD．being alkaline．The secondm~structure of QM protein contmned 6 -helices and 10 p-folding，others were random coils．QM gene expressed in sheep spleen，brain tissue and bone tissue better，while that also expressed in 7 other tissues．The expression quaritity of QM gene in diseased tissues was higher than that in normal tissues．Among 14 species，evolutionary distance of QM gene between sheep and cattle was shoaest．『Conclusion]QM gene was related with tumor suppression，cel1 growth，differentiation，development，death，etc．and it had extremely highconservation． Key words Sheep；QM gene；In sillco Cloning；Expressed