mir-171 基因家族进化分析及靶基因预测 - 生命科学研究.pdfVIP

sk 19 卷 6 期 生命科学研究 灾燥造援员9 晕燥援6 圆园15 年 12 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Dec. 圆园15 miR-171 基因家族进化分析及靶基因预测 a a b* 王艳芳, 周瑞莲, 赵彦宏 (鲁东大学a. 生 科学学院; b. 农学院, 中国山东烟台264025) 摘 要: 运用生物信息学方法在miRBase 中搜索植物miR-171 基因家族的序列, 分析miR-171 序列的进化特 征并预测其靶基因 结果表明, 在38 种植物中共搜索到219 条miR-171 序列, 大部分miR-171 基因都存在于 基因间隔区 进化分析表明, miR-171 基因家族的进化与物种进化关联不大 采用3 个miRNA 靶基因预测软 件对大豆和玉米miR-171 基因的靶基因进行预测, 发现了miR-171 可能参与生长因子尧转录因子尧蛋白酶等的 调控, 作用范围非常广泛 关键词: miR-171; 基因家族; 分子进化; 靶基因预测 中图分类号: Q752 文献标识码: A 文章编号: 1007-7847(2015)06-0479-05 Molecular Evolution of miR-171 Gene Family and Prediction of Their Target Genes 1 1 2* WANG Yan-fang , ZHOU Rui-lian , ZHAO Yan-hong (a. School of L if e Science ; b. School of A griculture, L udong Unive rs ity, Yantai 264025, Shandong, China) Abstract: Sequences of miR -171 gene family were downloaded in miRBase by bioinformatics method. Molecular evolution of miR-17 1 gene family was analyzed and their target genes were predicted. The results showed that 219 sequences of miR-17 1 genes were found in 38 plant species totally. Most of the miR-171 genes were located in the intergenic region (IGR). There was no closely relationship found between molecu lar evolution of miR-17 1 gene family and the evolution of plants. Target genes of miR-17 1 gene family were predicted by three target gene prediction softwares in