浙江省幽门螺杆菌cagA、vacA基因型的流行病学研究.pdfVIP

· 专题硼奔 · J Med Res，June 2008，Vo1．37 No．6 浙江省幽门螺杆菌cagA、vacA基因型的流行病学研究 林 朗 杨 恩 张红河 杨宁敏 摘 要 目的 调查浙江地区幽门螺杆菌(helicobacter pylori，Hp)基因型的分布及其与临床疾病的关系。方法 采用特异 引物聚合酶链反应(PCR)分析262株幽门螺杆菌的vacA基因多态性分布特点，并对其进行初步统计分析。结果 浙江8个地区 分离培养的Hp菌株262株，VacA mlb基因型占27．10％(71／262)；VacA m2基因型占64．89％(170／262)；VacA mlb—m2基因型 占4．20％(11／262)；不同地区间VacA s区基因型和 cA m区基因型分布差异无统计学意义(P0．05)。慢性胃炎分离株中 VacA mlb基因型占26．47％(27／102)；VacA m2基因型占66．67％(68／102)；VacA mlbm2基因型占2．94％(3／102)。消化性溃疡 分离株中 VacA mlb基因型占29．41％(40／136)； cA m2基因型占61．76％(84／136)；VacA mlbm2基因型占3．68％(5／136)。胃 癌分离株中 VacA mlb基因型占16．67％(4／24)； m2基因型占75．o0％(18／24)；VacA mlbm2基因型占4．17％(1／24)。不同 临床疾病间VacA m区基因型分布差异无统计学意义(P0．05)。结论 浙江8个地区Hp菌株的优势基因型为cagA+、va— eAsl／m2，vacA的s区和m区的分布与分布个体的临床类型无关。 关键词 幽门螺杆菌 vacA基因型 流行病学 Molecular Epidemiologic Study of VacA Genotypes of Helicobacter Pylori in Zhejlang Province． Lin 凡g，yn凡g En，Zhang Honghe， yn凡g Ningmin．Cangnan County Hospital，zh~jiang 325800，China Abstract 0bjective To investigate the the distribution of Helicobacter pylori(H．pylori)vacA gentypes and the relationship be· tween the presence of specific genotypes and clinical diseases in Zhejiang of China．Methods 262 Helicobacter pylori strains were colle· ted from 8 districts of Zhejiang，chromosome DNA was extracted and polymerase chain reaction(PCR)was carried out to determine the polymorphism of vacA and cagA with specific primers．PCR results were analyzed statistically according to their isolated original and clini· cal outcomes．Results VacA mlb，vacAm2 and vacAmlbm2 were found positive in 27．10％ ，4．89％ and 4．20％ of the 262 H．Pylori strains respectively．There was no significant difference in vacA genotypes among different districts of Zhejiang．26．47％(27／102)of vacA mlb,66．67％(68／102)vacAm2 and 2．94％(3／102)vacAmlbm2 Helicobacter pylori strains were isolated from chronic gastritis； 29．41％(40／136)of vacA