细胞膜的损伤作用.PDF

※基础研究 食品科学 2013, Vol.34, No.15 27 绿色木霉菌发酵液萃取物对金黄色葡萄球菌 细胞膜的损伤作用 * 杨鹏斌，于 新 ，杨 静，刘文朵，刘 丽 (仲恺农业工程学院轻工食品学院，广东 广州  510225) 摘  要：研究绿色木霉菌发酵液的萃取物对金黄色葡萄球菌的抑制活性，及其对细胞膜的损伤作用。通过抑菌实 验、细胞裂解率测定、细胞膜渗透性测定、SDS蛋白谱变化等对绿色木霉菌发酵液萃取物的抑菌活性和其对 细菌细胞膜损伤的作用进行研究。石油醚、乙酸乙酯以及正丁醇萃取物对金黄色葡萄球菌均有抑制活性，其中，正 丁醇萃取物的抑菌活性最强，抑菌圈直径(25.27 ±0.65)mm ，最低抑菌质量浓度(MIC)为0.625mg/mL 。经过正丁醇萃 取物处理后，菌体细胞裂解率增加，细菌培养液的电导率、蛋白质以及还原糖含量显著增加，SDS分析证明 正丁醇萃取物可以阻碍金黄色葡萄球菌细胞膜蛋白质的正常表达。正丁醇萃取物可破坏金黄色葡萄球菌细胞膜的结 构，导致细胞膜通透性增加，改变细胞膜功能，从而抑制其生长。 关键词：绿色木霉菌；发酵液；萃取物；金黄色葡萄球菌；细胞膜；损伤 Effect of Extract from Trichoderma viride Fermentation Broth on Cell Membrane Injury of Staphylococcus aureus * YANG Peng-bin ，YU Xin ，YANG Jing，LIU Wen-duo，LIU Li (College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China) Abstract ：This study investigated the antibacterial activity of different extracts from T. viride fermentation broth and its damage to the cell membrane of Staphylococcus aureus. The antibacterial activity was evaluated by Oxford plate method, the proportion of Staphylococcu aureus cell lysis was determined and the damage to the cell membrane was evaluated by determining membrane permeability and SDS. Petroleum extract, ethyl acetate extract and n-butanol extract from T. viride fermentation broth exhibited antibacterial activity, especially n-butanol extract (d = (25.27 ±0.65) mm, MIC = 0.625 mg/mL). After treated by n-butanol extract, the proportion of cell lysis, electric conductivity, protein and reducing sugar content of Staphylococcus aureus culture suspension increased significantly. SDSassay demonstrated that n-butan