A SPECTROPHOTOMETRIC METHOD FOR （分光光度法测定了）.pdf

A SPECTROPHOTOMETRIC METHOD FOR MEASURING THE BREAKDOWN OF HYDROGEN PEROXIDE BY CATALASE* BY ROLAND F. BEERS, JR., AND IRWIN W. SIZER (From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts) (Received for publication, September 24, 1951) Several methods have been developed for following the breakdown of hydrogen peroxide catalyzed by catalase, but these either have not been sufficiently quantitative or have not proved rapid enough to yield reliable data during the critical 1st or 2nd minute of the reaction. Chemical pro- D o cedures in which residual peroxide is titrated with permanganate (l-3) or w n l an excessof permanganate is measured calorimetrically (4) are accurate o a d except for reaction times of less than a minute, although Lemberg and e