屏障系统内的环境微生物控制与其生产种群的来源 - 中国试验动物学报.DOC

应用过氧化氢发生器对实验动物屏障环境及设施消毒效果的观察 杨文(，陈健茂，李红兵，郎多勇，冉林武 （宁夏医科大学实验动物中心，银川 750004） 【摘要】目的 观察过氧化氢（H2O2）蒸汽对屏障环境内表面、台面以及空气的消毒效果。方法 用BIOQUELL Z过氧化氢气体发生器将浓度为30%的H2O2液体分别按6 mL/m3、9 mL/m3和12 mL/m3的用量进行汽化熏蒸处理，作用60 min，检测屏障环境空气落下菌数和表面细菌数。结果 6 mL/m3用量能够杀灭二更、清洁走廊、洁存间、次清洁走廊和缓冲间等空气落下菌较少区域的空气细菌；9 mL/m3用量可以完全杀灭动物饲养室的空气细菌，但不能完全杀死动物饲养室内表面细菌；而12 mL/m3用量可以完全杀死动物饲养室内表面细菌。结论 H2O2蒸汽消毒适合屏障环境微生物的控制，操作简单，效果明显。 【关键词】过氧化氢蒸汽；实验动物；屏障环境；消毒 【中图分类号】Q95-331 Observation on Sterilization Effects of Hydrogen Peroxide Vapor in Laboratory Animal Barrier Environment YANG Wen, CHEN Jian-mao, LI Hong-bin, LANG Duo-yong, RAN Lin-wu (Laboratory Animal Center, Ningxia Medical University, Yin Chuan 750004, China) 【Abstract】 Objective To observe the sterilization effects of hydrogen peroxide vapor on hard surface and air in laboratory animal barrier environment. Methods BIOQUELL Z sterilizer continuously sprayed 30% hydrogen peroxide vapor by 6 mL/m3、9 mL/m3 and 12 mL/m3 for 60 min at different positions in laboratory animal barrier environment. The numbers of falling bacteria and bacteria on the surface in barrier environment were detected. Rusults The falling bacteria in second changing rooms, clean corridor, storage room, dirt corridor and buffer room were killed completely by amount of 6 mL/m3 H2O2, and the falling bacteria in animal feeding room were killed completely by amount of 9 mL/m3 H2O2, but the bacteria on the surface in barrier environment were not completely killed. The bacteria on the surface in barrier environment were completely killed by amount of 12 mL/m3 H2O2. Conclusion Hydrogen peroxide vapor has very good sterilization effects on laboratory animal barrier environment and housing facilities. 【Keywords】Hydrogen Peroxide Vapor; Laboratory Animal; Barrier Environment; Sterilization 目前，用于屏障环境内空气和表面消毒的方法主要有：乙醇擦拭，过氧乙酸擦拭，含氯消毒剂擦拭以及紫外灯照射等方法[1,2]。这些常规方法副作用和损坏性明显，而且消毒不彻底。过氧化氢（H2O2）因具有氧化还原作用而具有杀菌效果，可根除细菌、病毒、真菌和芽孢，是一种广谱微生物消毒和灭菌方式，而且易分解、对环境无毒[3]。因此，本研究旨在观察H2O2蒸汽对屏障环境内表面、台面以及空气的消毒效果。现将结果报告如下。 1 材料和方法 1.1