乳腺浸润性导管癌Survivin及Survivin mRNA表达探究.docVIP

乳腺浸润性导管癌Survivin及Survivin mRNA表达探究【摘要】 目的 检测Survivin在乳腺浸润性导管癌病变中的表达，探讨其与乳腺浸润性导管癌病变的关系。方法 在48例乳腺浸润性导管癌病变组织和其癌旁组织中分别应用原位细胞凋亡检测(TuNEL)试剂盒测定原位细胞凋亡指数(Apoptosis Index，AI)，应用免疫组化法检测Survivin，应用原位杂交法检测Survivin mRNA表达。结果 Survivin及Survivin mRNA在乳腺浸润性导管癌中表达显著升高（P＝0.001 6，P＝0.000 0）；Survivin及Survivin mRNA灰度值与AI呈正相关（r＝0.533，P＝0.005；r＝0.455，P＝0.003）。结论 Survivin及Survivin mRNA表达升高可能参与乳腺浸润性导管癌的发生发展过程，检测Survivin及Survivin mRNA对于乳腺浸润性导管癌与良性组织判别具有临床参考意义。 【关键词】 乳腺癌；细胞凋亡；Survivin Significance of Survivin and Survivin mRNA in breast carcinoma LIN Hua-de，LIANG Hua-sheng，HUANG Yu.Department of General Surgery，the People＇s Hospital of Pingnan County，Guangxi 537300,China 【Abstract】 Objective To study the relationship between Survivin and breast carcinoma by combining assays of Survivin and Survivin mRNA in breast tissue.Methods Apoptosis Index (AI) was evaluated by TUMEL method，the expression of Survivin and Survivin mRNA were detected by immunohistochemistry and In Situ Hybridization in 48 breast carcinoma cases and those tissues beside carcinoma.Results The higher level of Survivin and Survivin mRNA were found in breast carcinoma compared with tissues beside carcinoma（P＝0.001 6，P＝0.000 0）.There was positive relationship between the expression of Survivin，Survivin mRNA and AI（r＝0.533，P＝0.005；r＝0.455，P＝0.003）.Conclusion Survivin may participate in proceeding of breast carcinoma，and it is suggested that combined detection of Survivin and Survivin mRNA may have important referent significance on the differentiation of benign and m alignant breast tissue. 【Key words】 Breast carcinoma；Cell apoptosis；Survivin 乳腺浸润性导管细胞癌的鉴别，既往研究多局限于单纯癌性指标的检测，而结合各种癌性指标与相关细胞增殖或凋亡等方面进行分析研究却很少见。因而，我们对48例乳腺浸润性导管细胞癌组织分别应用原位细胞凋亡检测(TuNEL)试剂盒测定原位细胞凋亡指数(Apoptosis Index，AI)，免疫组化法检测Survivin，原位杂交法检测Survivin mRNA表达情况，旨在探讨其在乳腺浸润性导管细胞癌发生与发展过程中的作用及其意义，亦可寻找其联合检测在乳腺浸润性导管癌病变诊断中的应用价值。 1 材料与方法 1.1 材料 组织标本来源于广西平南县人民医院、广西医科大学第九附属医院、广西人民医院2004～2006年手术切除，经病理检查证实为乳腺浸润性导管癌的病例共48例，均为女性，平均年龄（42.5±10.2）岁；均为广西籍；术前无化疗或其他药物