兔腹主动脉内膜损伤后血管狭窄模型建立及评价.docVIP

兔腹主动脉内膜损伤后血管狭窄模型建立及评价【摘要】 目的为了探讨再狭窄发生机制和预防措施，研究建立兔动脉粥样的狭窄动物模型及其评价的方法。方法 采用兔腹主动脉内膜剥脱术加含1．5%高胆固醇的高脂饮食来建立动脉粥样的狭窄动物模型，并采用血管彩色超声和血管造影检查测出狭窄指标，对动脉粥样的狭窄动物模型进行评价。结果 采用兔腹主动脉内膜剥脱术加含1．5%高胆固醇的高脂饮食所建立动脉粥样的狭窄动物模型，经血管彩色超声检查血管的狭窄程度、参照血管内径、血管狭窄段最小内径分别为（56．89±1．05）%、（2．940±0．032） mm 、（1．267±0．032） mm；和血管造影检查的上述指标［（57．74±1．089）%、（2．945±0．031） mm、（1．244±0．034） mm］相比差异无统计学意义（P0．05）。结论 本实验通过内膜剥脱术加高脂饮食在短时间内成功地建立了兔动脉粥样的狭窄动物模型，并认为血管彩色超声检查是一种较理想的评价该动物模型的方法。 【关键词】 动脉粥样硬化；狭窄；超声检查；血管造影；模型 Establishment and evaluation of rabbit restenosis model after angioplasty XU Wen-ke, GAO Chuan-yu. Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052,China 【Abstract】 Objective To establish and evaluate an animal model to study the mechanism of restenosis for preventing and treating restenosis. Methods The rabbits were underwent intimal denudation in the abdominal artery and constantly fed with a 1．5% cholesterol diet in order to establish restenosis model.And in order to evaluate the established restenosis model, ultrasonographic image and angiography on abdominal aorta were performed in 12th weeks after balloon injuryto measure the degree of stenosis.Results By Ultrasonographic image showed that, in the abdominalis aorta of experimental group, the degree of stenosis was (56．89±1．05)%, the reference lumen diameter was (2．940±0．032) mm, and the minimum lumen diameter was (1．267±0．032) mm ; The results of experimental group were showed by angiography as follows: (57．74±1．089)%, (2．945±0．031) mm, (1．244±0．034) mm；the results between the two estimated evaluatal methods had no significant difference，（P0．05）．Conclusion The animal model of stenosis could be successfully established in a short time by balloon endothelial denudation of abdominal aorta in cholesterol-fed rabbits. Doppler ultrasonography appear suitable for measuring the degree of stenosis of the abdom final aorta when compared to angiography. 【Key words】 Atherosclerosis; Stenosis; Ultr