半定量RT-PCR法测定TRAIL、survivin在胃癌组织中表达及其意义.docVIP

半定量RT-PCR法测定TRAIL、survivin在胃癌组织中表达及其意义[摘要]目的 检测肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factor-related apoptosis-inducing ligand,TRAIL）及抑制凋亡基因家族成员survivin在胃癌组织中的表达,并探讨其意义。方法 利用半定量逆转录聚合酶链反应（RT-PCR）法检测82例胃腺癌及对应的正常胃组织中TRAIL和survivin的表达。 结果 TRAIL在正常胃组织中的表达阳性率及相对表达量均明显高于癌组织(P0.05)；而survivin的表达则与TRAIL表达相反，正常胃组织中不表达，在胃癌组织中的表达阳性率为62.2%。TRAIL和survivin表达量与肿瘤的分化程度有关，分化程度越低，TRAIL mRNA表达量越低，而survivin mRNA表达量越高，高分化胃腺癌与低分化胃腺癌间差异有统计学意义(P0.05)。 结论 TRAIL和survivin在胃癌凋亡过程中起着重要的作用，TRAIL可诱导凋亡，survivin则抑制凋亡发生。? [关键词] 胃癌;肿瘤坏死因子相关凋亡诱导配体；survivin；逆转录聚合酶链反应 ? [中图分类号] R735.2;Q786 [文献标识码] A [文章编号] 1671-7562（2007）02-０１２５-０４?? Detection of TRAIL and survivin in gastric ?carcinoma by semiquantitive RT-PCR?? WANG Xiao-hong1,WANG Suo-rong1,ZHOU Qing2，ZHU Chun-mei1,CAO Suo-yu?1 (1.Department of Gastroenterology,the People?s Hospital of Danyang，Danyang 212300，China;?2.Department of Pathology,the Affiliated Hospital of Nantong University，Nantong 226001，China)?? Abstract:Objective To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)and survivin in gastric carcinoma.Methods Expression of TRAIL and survivin were assayed by semiquantitive reverse transcriptase polymerase chain reaction(RT-PCR)in 82 cases of gastric carcinoma and normal gastric tissues.Results The positive rate and expression quantity of TRAIL in normal tissues were higher than those in cancer tissues(P0.05).However，the survivin was only expressed in cancer tissues,the positive rate was 62.2%.The expression quantity of TRAIL and survivin was correlated with histological grade，the expression of TRAIL was higher in well-differentiated gastric adenocarcinoma than that in poorly differentiated gastric adenocarcinoma while the expression of survivin was lower(P＜0.05).Conclusion TRAIL can induce apoptosis and survivin can depress the happening of apoptosis，which may be one of the mechanisms by which gastric carcinoma develops.? Key words:gastric carcinoma;tumor necrosis factor-related apo