土牛膝中齐墩果酸-牛膝多糖提取及鉴定.docVIP

土牛膝中齐墩果酸\牛膝多糖提取及鉴定【摘要】 目的 从土牛膝中提取齐墩果酸和多糖并进行定性定量分析。方法 土牛膝水煮液经水浴、酸水解、加碱煮沸后,乙醇回流、抽滤,60℃烘干精制得到齐墩果酸;土牛膝的乙醇热回流后再水提,经过浓缩、沉淀、除杂、抽滤、洗涤、低温干燥得到牛膝多糖。用薄层色谱法和分光光度法进行定性定量分析。结果 齐墩果酸的提取率为0.88%;牛膝多糖的提取率为7.2%。结论 通过含量测定结果与提取率对比,本实验方法可行。 【关键词】 土牛膝;齐墩果酸;牛膝多糖;提取与鉴定 Extracting and Identification of OA and ABPS of local achyranthes aspera HUANG Zhi-fang,HUANG Zhi-hong, HU Ji-lin,et al. Department of Chemistry and Life Science, Xiangnan University, Chenzhou 423000,China 【Abstract】 Objective To extract oleanolic acid(OA) and achyranthes bidentata polysaccharides(ABPS) from local achyranthes aspera, and analyze quantitatively and qualitatively. Methods The oleanolic acid(OA) was obtained from the decoction of local achyranthes aspera through the process of washing, acid hydrolysising, adding alkali and boiling; ethanol refluxing, vacuum filtrating and 60℃ drying. After the ethanol hot refluxing and aqueous extracting, the achyranthes bidentata polysaccharides (ABPS) are obtainedthrough the process of condensing, sedimenting, impurities removing, vacuum filtrating, washing, cooling and drying. Ultraviolet spectroscopy and TCL are adopted for qualitative and quantitive analysis. Results The extracting rate of OA and ABPS were 0.88% and 7.2% respectively. Conclusion The experimental method of comparising the content determination and the extracting rate is easy and feasible. 【Key words】 Local achyranthes aspera;Oleanolic acid(OA);Achyranthes bidentata polysaccharides(ABPS);Qualitative and quantitive analysis 土牛膝,别名倒扣草,《中国药典汇编》对土牛膝有如下记载:药性:苦、凉。归肺、脾、肝、膀胱经。药效: 败毒抗癌、逐淤除痹,消炎利尿。现代医学研究证明,土牛膝具有改善机体微循环,调整新陈代谢,改善免疫功能等作用。具有明显的抗炎抗菌作用、镇痛作用、抗衰老等[1-3]作用。近年来有研究表明与牛膝含有成分的齐墩果酸、牛膝多糖具有治疗糖尿病的功效[4-6],但土牛膝提取物在治疗糖尿病方面的研 究尚无报道。以本地野生土牛膝为原料经过提取、分离、过滤、干燥得到土牛膝提取物齐墩果酸、牛膝多糖,将对土牛膝提取物治疗糖尿病的研究具有重要意义。 1 材料 1.1 土牛膝 湖南郴州郊外野生自己采集、洗净、除去残留芦头、切段、晒干。 1.2 试剂和仪器 齐墩果酸标准品,优级纯,什邡市巨邦植物原料有限公司;葡萄糖,其余试剂均为市售分析纯。电子天平,上海精密仪器仪表有限公司;低压型抽虑装置,唐山市玻璃仪器厂;722型分光光度计,上海天翔光学仪器有限公司;硅胶板:5～12 cm北京市昌平石鹰化工厂。 2 方法与结果 2.省略 2.