基质金属蛋白酶-7在喉癌中表达及及肿瘤侵袭转移、血管生成关系.docVIP

基质金属蛋白酶-7在喉癌中表达及及肿瘤侵袭转移、血管生成关系【摘要】 目的 观察基质金属蛋白酶-7（MMP-7）蛋白在喉癌中的表达，探讨其表达与肿瘤侵袭转移、血管生成及预后的关系。方法 采用免疫组化方法检测MMP-7在48例喉癌标本中的表达水平，运用χ2检验、直线回归分析、乘积极限法、对数秩检验进行统计学分析。结果 MMP-7在喉癌中的阳性表达率为81．25%（39/48），明显高于癌旁正常喉黏膜上皮的18．75%（6/48）；MMP-7与患者年龄、肿瘤病理分化程度无关，与喉癌原发灶浸润深度、区域淋巴结转移范围和临床分期相关；喉癌MMP-7蛋白表达水平与肿瘤微血管密度呈线性依存关系，其回归系数为0．60；低表达（阴性或弱阳性）MMP-7的喉癌标本术后3年和5年的生存率分别为82．38%、73．23%，明显高于高表达（阳性或强阳性）MMP-7的喉癌标本术后3年和5年的生存率分别为66．42%、44．28%。结论 MMP-7在喉癌中呈阳性表达，其表达与肿瘤侵袭转移程度呈正相关系，与喉癌微血管密度呈线性依存关系，高表达MMP-7的喉癌预后较差。 【关键词】喉肿瘤；基质金属蛋白酶-7；免疫组织化学；肿瘤转移；血管生成；预后 Expression of matrix metalloproteinase-7 in laryngeal carcinoma and it?s significance in cancer metastasis and Angiogenesis XU Wei-hua，CHEN Ren-hui,FU Ya-feng,et al. Department of Otolaryngology-Head and Neck Surgery,The Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China 【Abstract】 Objective To observe the expression of matrix metalloproteinase-7 (MMP-7) in laryngeal carcinoma and it?s significance in metastasis angiogenesis and prognosis.Methods Immunochemistry were used to detect the expression of MMP-7 in laryngeal carcinoma.Chi-square test,linear regression,Kaplan-Meier method and log-rank test were applied in statistic analysis.Results Expressive rate of MMP-7 in laryngeal carcinoma was 81．25%,obviously higher than 18．75%in normal laryngeal epithelial.MMP-7 expression was not coherent with patients age and histology classification.But the relationship was existed between MMP-7 expression and laryngeal cancer invasive depth and rational lymphatic metastasis and TNM staging.Linear dependency was exist between MMP-7 expression and tumor microvessel density (MVD).The 3 years and 5 years survival rate of low MMP-7 express (negative or slight positive) laryngeal carcinoma were 82．38%and 73．23%,obviously higher than 66．42%and 44．28%in high express (positive or high positive) group.Conclusion MMP-7 express in laryngeal carcinoma.The expression are related with cancer invasion and metastasis.Linear dependency was exist between M