慢性淋巴细胞白血病免疫表型探究.docVIP

慢性淋巴细胞白血病免疫表型探究［摘要］ 目的 结合ZAP-70、CD38探讨免疫表型在B慢性淋巴细胞白血病（B-CLL）诊断和预后评价中的应用价值。方法采用多参数流式细胞术检测45例B-CLL患者CD5、CD10、CD19、CD20、CD22、CD23、FMC7、CD38及ZAP-70 的表达。结果45例B-CLL均高表达CD19、CD20，其中有43例（95.6%）表达CD5,42例（93.3%）表达CD23，30例表达CD22（66.7%），无一例表达FMC7和CD10。将所有病例分为两组，ZAP-70?+CD38?+ /ZAP-70?-CD38?-组占84.4%（38/45）,ZAP-70?+CD38?-/ZAP-70?-CD38?+占15.6%（7/45），两组所占比例差异有统计学意义（P0.05）。结论 同时表达CD5、CD23、CD19是诊断B-CLL的特征性免疫表型,CD5?-见于不典型B-CLL，CD38和ZAP-70的表达存在相关性。? ［关键词］ 慢性淋巴细胞白血病；流式细胞术；ZAP-70；CD38? [中图分类号]R733.72 [文献标识码]A [文章编号]1671-7562（2007）02-0129-04?? Study of immunophenotype in chronic lymphocytic leukemia?? WU Yu-jie1,CHEN Bao-an1,LI Jian-yong2，XU Wei2，YANG Hui2， (1.Department of Hematology,Zhongda Hospital,Southeast University,Nanjing 210009,China; 2.Department of ?Hematology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)?? Abstract:Objective To investigate the characteristics of immunophenotype of B chronic lymphocytic leukemia (B-CLL) and to evaluate the value of ZAP-70 and CD38 in diagnosis and prognosis of B-CLL.Methods The expression of CD5，CD10，CD19，CD20，CD22，CD23，FMC7,CD38 and ZAP-70 of 45 B-CLL patients were determined by four-color flow cytometry(FCM).Results Forty-five B-CLL patients were all over-expression of CD19 and CD20,among them,CD5,CD23,CD22 were expressed in 43,42,30 patients respectively,no case was seen expression of FMC7 and CD10.All the cases were divided into two groups,84.4%(38/45) was in ZAP-70?+CD38?+ /ZAP-70?-CD38?-group,15.6%(7/45) was in ZAP-70?+CD38?-/ZAP-70?-CD38?+group.There was significant difference betweenthe ratio of the two groups(P0.05).Conclusion The expression of CD5,CD23,CD19 simultaneously is the characteristics of immunophenotype of B-CLL.CD5 is existed in non-typical B-CLL.ZAP-70 and CD38 are concordantly expressed in B-CLL.? Key words:chronic lymphocytic leukemia;flow cytometry;ZAP-70;CD38? (Modern Medical Journal,2007,35:129-132)?? 慢性淋巴细胞白血病（CLL）是一种低度恶性的小淋巴细胞异质性疾病，以外周血（PB）及骨髓（B