水提-醇沉法提取大黄多糖工艺优化探究.docVIP

水提-醇沉法提取大黄多糖工艺优化探究【摘要】 目的 确定大黄多糖的最佳提取条件。方法 以大黄多糖的含量为指标,以提取温度、提取时间、提取次数、料液比为因素,利用正交试验法进行试验设计,对传统水提-醇沉法提取大黄多糖的工艺进行优化研究。结果 大黄粗多糖得率最高的提取条件是:提取时间3 h,提取温度95℃,料液比1?30;大黄粗多糖纯度最高的条件是:提取时间1 h,提取温度95℃,料液比1?10;乙醇沉淀大黄多糖的最佳浓度是80%。结论 本方法实验结果可作为提取大黄多糖的工艺制定的依据。 【关键词】 大黄;多糖;水提-醇沉法;工艺研究 Studies on the process of rhubarb thick polysaccharide tradition through the traditional water solution and alcohol sedimentation SUN Ying,JI Yue-zhi,MA AI-ming,et al. Changchun Medical College, Changchun 130031,China 【Abstract】 Objective To determine the optimum extracting conditions of rhubarb polysaccharide.Methods By orthogonal test, take the content of rhubarb polysaccharide as standards, extraction temperature, extraction time,extraction times and ratio of material to liquid as factors, to optimize and study the process of extracting rhubarb polysaccharide through the traditional water solution and alcohol sedimentation.Results The optimum extraction condition of rhubarb thick polysaccharide has been established as follows:extraction time was 3.0 hours,extraction temperature was 95 ℃, and ratio of material to liquid 1:30.The optimum purity condition of rhubarb thick polysaccharide had been established as follows:extraction time was 1.0 h,extraction temperature was 95 ℃, and ratio of material to liquid 1:10.The best density of ethanol depositing rhubarb polysaccharide was 80%. Conclusion The experimental result of this method can be the basis of extracting rhubarb polysaccharide process. 【Key words】 Rhubarb;Polysaccharidek;Water solution and alcohol sedimentation;The process study 大黄为廖科植物掌叶大黄(RheumpalmatumL.),唐古特大黄(Rheum tanguticumMaxim. ExBalf)或药用大黄(RheumofficinaleBaill)的干燥根及根茎,作为传统中药在临床上应用具有悠久的历史,其功效为泻下、健胃、清热解毒等。近年来,药理实验表明大黄多糖具有改善血脂[1]、降血糖[2]、抗衰延年[3]等作用,并可以通过提高肌体免疫力达到抗肿瘤的作用[4]。由于大黄多糖具有多方面的生理活性,而且毒性低,性质稳定,营养价值高等特点,因此,越来越引起人们的重视。本文为了更好的利用大黄多糖,有效的开发大黄多糖资源,仅以大黄多糖的含量为指标,采用单因素考察和正交实验法进行试验设计,对传统的水提-醇沉法提取大黄多糖的工艺进行系统研究,确定最佳提取工艺,为大规模工业生产大黄多糖提供科学依据。 1 仪器与试药 1.1 仪器 U1901双光束紫外可见分光光