水稻品种Lemont响应低氮培养及共培稗草的上调表达基.PDFVIP

2012 9 20 9 Chinese Journal of Eco-Agriculture, Sep. 2012, 20(9): 1185−1190 DOI: 10.3724/SP.J.1011.2012.01185 “Lemont” * 方长旬 许铁城 黄力坤 王清水 何海斌 林文雄** ( 350002) 营养匮乏和伴生杂草是水稻种植过程中的常见影响因素。本研究应用抑制消减杂交(SSH)技术, 分别 研究低氮、稗草条件下, 非化感水稻品种“Lemont” 的上调表达基因。结果显示, 低氮条件下, “Lemont”水稻中 参与生长调控的生长素响应蛋白, 参与抗逆防御的类NBS-LRR 蛋白、过氧化氢酶、金属硫因蛋白, 以及参与 蛋白质代谢相关蛋白的编码基因上调表达。稗草共培下, 编码NADH 脱氢酶、细胞色素氧化酶、ATP 依赖性 RNA 解旋酶等与植物生长相关的基因, 与抗逆防御相关的几丁质酶和糖基水解酶基因, 以及与信号转导相关 的锌指蛋白及组氨酸激酶基因增强表达。研究结果表明, 非化感水稻“Lemont” 能够通过调节抗逆以及生长调 节相关基因的表达, 从而响应不同的胁迫。 水稻 低氮条件 稗草共培 基因表达 : S314 : A : 1671-3990(2012)09-1185-06 Analysis of up-regulating genes of “Lemont” rice accession in response to low nitrogen supply and accompanying barnyardgrass FANG Chang-Xun, XU Tie-Cheng, HUANG Li-Kun, WANG Qing-Shui, HE Hai-Bin, LIN Wen-Xiong (Institute of Agroecology, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstract Nutrient deficiency and the accompanying weeds are generally the influencing factors of rice growth. In this research, suppression subtractive hybridization (SSH) was used to investigate up-regulating genes in non-allelopathic “Lemont” rice accession respectively in response to low nitrogen condition and accompanying barnyardgrass. The results showed that genes encoding aux- in-responsive protein which involved in plant growth and cell cycle regulation, NBS-LRR-like protein, catalase, metallothionein-like protein that involved in stress resistance, and protein metabolism relevant genes were up-regulated under low nitrogen condition. When rice was accompanied with barnyardgrass, genes encode NADH dehydrogenase, ATP-