葡萄糖干预对电磁辐射引起的大鼠学习记忆功能 - 第三军医大学学报.doc

葡萄糖干预对电磁辐射所致大鼠学习记忆功能障碍的影响 卢永辉，何旻蒂，张彦文，周 舟，张广斌，余争平，钟 敏 (400038 重庆，第三军医大学劳动卫生学教研室，电磁辐射医学防护教育部重点实验室) [摘要] 目的 观察平均功率密度为30 mW/cm2的连续微波辐射对大鼠学习记忆功能和海马葡萄糖摄取（Glucose uptake）的影响及葡萄糖干预作用。方法 成年雄性SD大鼠48只，其中18只用于Morris水迷宫实验，分为对照组、暴露组、葡萄糖干预暴露组，每组6只；暴露组、葡萄糖干预暴露组接受平均功率密度30 mW/cm2的微波连续暴露28 d，每天20 min，暴露结束后Morris水迷宫检测大鼠学习记忆功能变化，Morris水迷宫训练前30 min，葡萄糖干预暴露组大鼠按500 mg/kg体质量腹腔注射葡萄糖，对照组和暴露组腹腔注射生理盐水。另30只用于海马葡萄糖摄取实验，分为对照组，暴露7、14、21、28 d组，每组6只，分别接受0、7、14、21、28 d平均功率密度为30 mW/cm2的微波暴露，各组暴露期满后，液体闪烁计数法检测大鼠海马组织对3H-2脱氧葡萄糖（3H-2DG）的摄取量变化。结果 平均功率密度为30 mW/cm2的微波连续暴露28 d后，暴露组大鼠第4、5、6、7天逃避潜伏期较对照组延长（P＜0.050.05）；暴露组大鼠在目标象限停留时间（18.66±2.77）s和跨越虚拟平台次数（1.6±0.54）分别较对照组[（30.75±8.02）s，2.8±0.83）]下降（P＜0.050.05）。微波暴露21 d组（3959±390）和28 d组（3764±192）大鼠海马对3H-2-D-Glucose摄取量较对照组（5284±711）显著减少(P＜0.05)electromagnetic radiation Lu Yonghui, He Mindi, Zhang Yanwen, Zhou Zhou, Zhang Guangbin, Yu Zhengping, Zhong Min (The Department of Occupational Hygiene, Key Laboratory of Electromagnetic Radiation Protection, The Third Military Medical University, Chongqing, 400038, China) [Abstract] Objective To investigate the change of hippocampal glucose uptake after whole-body exposure to 30mW/cm2 microwave, and explore the effect of glucose on learning and memory deficits induced by electromagnetic radiation (EMR) in rats. Methods 18 adult male SD rats were randomly divided into control group (CON), microwave exposure group (MW), microwave exposure and glucose injection group (MW+GLU), 6 per group. MW and MW+GLU group received 30mW/cm2 microwave exposure for 28 days, then the learning and memory of the rats were detected by Morris water maze. 30min before training, the MW+GLU group were administered intraperitoneally 500 mg/kg of glucose every day from the start of training. 30 rats received 30mW/cm2 microwave exposure 20min once a day for 0, 7, 14, 21, 28 days respectively, 6 per group, then hippocampal glucose uptake was determined by liquid scintillation counter. Results The escape Latency of MW group increased compared to CON (P