槟榔黄化病组织RNA提取方法的比较.PDF

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 10 8 RNA 1, 2 1, 2 3 1, 2 3 周亚奎 杨云 隋春 甘炳春 魏建和 1 ( , 571533; 2 , 571533; 3 , 100193) : 为了从发生黄化病的槟榔茎叶花中提取到完 的 RNA, 采用了 CTAB 法Trizol法和异硫氰酸胍法提取槟榔 黄化病组织 RNA从 RNA 的完 性产率和纯度方面对这几种方法进行了比较, 结果表明, 异硫氰酸胍法所得RNA 完 性较 好, 条带清晰无明显降解, OD /OD 介于 18- 2. 0之间, RNA得率较高, 为 120 g/g 以上, 并能成功进行反转录制备 DNA, 260 280 表明该 RNA 可以进行后续分子生物学操作 : 槟榔 黄化病 RNA提取 异硫氰酸胍法 Comparison ofM ethods for Extracting Total RNA from Yellow Leaf Disease Tissue ofAreca catechu L. 1, 2 1, 2 3 1, 2 3 Zhou Y aku i Y ang Yun SuiChun Gan B ing hun W e i Jianhe 1 ( H a inan B ranch Institute of M ed ic ina l P lan,t Ch inese A cad emy of M ed ica l S ciences P eking Un ion M ed ica l Colleg e, W ann ing 571533 2H a inan P rov incia lK ey L abora tory of R esources Conserva tion and D ev elopm en t of S outh ern M ed icine, Wan ning 571533 3 Institute of M ed icin al P lan tD ev elopm en,t Ch inese A cad emy of M ed ica l S cienc es P eking UnionM ed ical Colleg e, B eij ing 100193) A bstrac:t In order to extra t pure and integrate RNA from stem, leaf and flower ofA reca catech u L. , theCTAB, Trizol and guan idine isoth io yanatem ethod w ere om pared. A ording to the integrity, produ tivity and purity of extra ted RNA, the guan id ine isoth io yanatem ethod w as them ost appropriatem ethod of RNA extra tion forA reca ca techu L. W ith this m ethod, the RNA bands were lear w ithout degradation, the ratio of OD260 /OD280w ere 1