鬼臼毒素醇质体的体外经皮渗透特性研究.PDF

·74 · 中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 1 期 2012 年 1 月 鬼臼毒素醇质体的体外经皮渗透特性研究 * 于燕燕，赵继会，冯年平 ，张永太，施晓琴 上海中医药大学中药学院，上海 201203 摘 要：目的 研究鬼臼毒素醇质体经皮渗透特性。方法 通过体外经皮渗透试验，比较鬼臼毒素在大鼠皮肤中的稳态透皮 速率和皮肤滞留量，分别考察以醇质体、酊剂、脂质体、30%乙醇混悬液以及鬼臼毒素与空白醇质体物理混合物作为载体， 鬼臼毒素经皮渗透特性。结果 鬼臼毒素醇质体的 12 h 皮肤滞留量为 8.17 μg/cm2 ，高于其他各组；稳态透皮速率小于鬼臼 毒素载药量为 0.5% 的脂质体组及乙醇混悬液组（P ＜0.05 ），与其他对照组无显著差异。结论 醇质体具有较大的皮肤滞留 量及较小的透皮速率。 关键词：鬼臼毒素；醇质体；稳态透皮速率；累积透过量；皮肤滞留量 中图分类号：R286.02 文献标志码：A 文章编号：0253 - 2670(2012)01 - 0074 - 04 Percutaneous permeation characteristic of podophyllotoxin ethosomes in vitro YU Yan-yan, ZHAO Ji-hui, FENG Nian-ping, ZHANG Yong-tai, SHI Xiao-qin College of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Abstract: Objective To study the percutaneous permeability characteristic of podophyllotoxin (POD) ethosomes in vitro. Methods Excised SD-rat abdomen skin was used as penetration barrier. The steady penetration rate and the skin residual amount were calculated to evaluate the percutaneous permeability of POD from ethosomes, tinctures, liposome, 30% hydro-ethanolic suspension, mixture of drug and blank ethosomes, respectively. Results The skin residual amount of POD in the ethosomes group was 8.17 μg/cm2 in 12 h, higher than those in the other groups. In addition, the steady penetration rate of POD in the ethosomes group decreased significantly compared with those in the liposome group with POD loading being 0.5%, and the hydro-ethanolic suspension group (P 0.05), while no obvious difference was seen among the ethosomes group and the other groups. Conclusion Higher skin residual amount and lower steady penetration rate are observed in the ethosomes group. Key words: podophyllotoxin (POD); ethosomes; steady penetration rate; cumulative transdermal amount; skin deposit amount 鬼臼毒素（podophyllotoxin ，POD ）是从小檗