植物组织培养中灭菌和无菌操作的几个问题（Several problems of sterilization and aseptic operation in plant tissue culture）.doc

植物组织培养中灭菌和无菌操作的几个问题（Several problems of sterilization and aseptic operation in plant tissue culture） Several problems of sterilization and aseptic operation in plant tissue culture Zhu guanglian Beijing university life sciences college, Beijing. In plant tissue culture, medium and apparatus The complete sterilization of the dish and the correct aseptic operation are the prevention of dyeing The key to the normal growth and differentiation of bacteria and tissue culture materials is The most basic and important technique in group work. Engaged in the Scientific workers related to group work should be in practice Can be flexible and clever according to the nature, conditions and requirements of the work The application of the principle, skilled, agile, and correctly carried out Operation. However, in teaching and research, there are always some Incorrect operation reflects a recognition of the concepts and principles The vagueness of knowledge. Lets talk about some of the problems Ive had And views, for the peer to learn from. Sterilization, sterilization and sterilization The term sterilization refers to the use of physical or chemical methods to kill All microorganisms on the surface of dead objects and inside. It is different from the Disinfection. Disinfection is a kind of health care that comes from medicine Shi, usually refers to the killing of harmful pathogenic microorganisms. In addition to bacteria is To remove liquids or gases by mechanical or other physical means The microorganisms in the microorganism achieve the purpose of sterile purification. Visible sterilization The object or medium after the bacteria is sterile and disinfected Objects may still contain a lot of microbes. In teaching and working It is often heard that sterilization is called sterilization, and the bacteria is said to be Sterilization, or sterilization, sterilization and antibacterial three different concepts Confused. This should be clarified. High temperature sterilization High temp