鲜蛋的检验（Inspection of eggs）.docVIP

鲜蛋的检验（Inspection of eggs） Technical regulations for the operation of bovine embryo transfer The embryo transfer of cattle has high technical requirement during the operation. Through the promotion of bovine embryo transfer technology in our county, the procedures for the operation of bovine embryo transfer are introduced as follows for reference. 1, the subject content and scope of application 1.1 the regulations provide the basic principles, requirements and methods for the technical operation of bovine embryo transfer. 1.2, this regulation applies to the standardized operation of bovine embryo transfer technology. 1.3 application of frozen embryos for bovine embryo transfer in this procedure. Selection criteria for 2 receptor cattle 2.1, 3~8 years of age, health, no disease, large physique, good fat. 2.2 reproductive performance was normal, more than 60d postpartum, and there was more than two normal natural estrus records. 2.3, good lactation performance, no dystocia history, do not use two artificial insemination, not pregnant cattle. Feeding and management of 3 receptor cattle 3.1, careful management, reasonable feeding, clean environment, forage, feed, drinking water should be fresh, add salt and necessary additives. 3.2 make up a certain number of cattle. Quanshe in order to prevent overcrowding, estrus observation. 3.3 farmers long in cows should pay attention to strengthening exercise. 3.4 prevent the stress reaction caused by feed and environment improvement before and after transplantation. Synchronization of estrus in 4 recipient cows 4.1 use of prostaglandin (PGF2), a single intramuscular injection, to induce estrus. The recipient of the corpus luteum at 8 to 15d, and the corpus luteum of the rectum, was injected PGF2 at a time. 4.2, two times PGF2 alpha treatment. Without considering the estrous cycle, PGF2 can be divided into 11d and two times before and after intramuscular injection. 5 estrus observation In principle, according to the acceptance of c