云芝菌发酵产漆酶及其对靛蓝脱色的研究-高校化学工程学报.PDF

21 1 No.1 Vol.21 2007 2 Journal of Chemical Engineering of Chinese Universities Feb. 2007 1003-9015(2007)01-0111-05 , , ( , 310027) (Coriolus versicolor) 20.0 g⋅L− 1 10.0 g⋅L− 1 C/N 20 Cu2+C 0.100 mmol⋅L− 1 1.500 mmol⋅L− 1 3.7 L 100 L⋅h− 1 144 h2474.2 IU⋅mL− 1 50 mg⋅L− 1 0.45%(V:V)40 min 94.8% Q554.9 TQ920.6 X703.1 X791 A Laccase Production by Coriolus versicolor Fermentation and its Decolorization Effect on Indigo Bule Dye GAO En-li, ZHANG Shu-jiang, XIA Li-ming (Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China) Abstract: The main technological parameters of the laccase production by Coriolus versicolor in submerged fermentation were studied. The corncob residue, a lignocellulosic waste from xylose manufactory, was found to be an effective carbon source for laccase production and the suitable corncob residue concentration to be used is 20.0 g⋅L− 1. The addition of 10.0 g⋅L− 1 glucose into culture medium could promote the growth of mycelium in the prophase and benefit the laccase synthesis during the anaphase. The most suitable C/N ratio of culture medium is 20 and adding appropriate amounts of Cu2+ (0.10 mmol⋅L− 1) and ascorbic acid (1.50 mmol⋅L− 1) could obviously enhance the laccase production. The experiments of producing laccase by Coriolus versicolor in a 3.7 − 1 L fermentor show that, with the above suitable condition and the most appropriate aeration rate of 100 L⋅h , after 144 h the