An Introduction to Fluorescence Spectroscopy（介绍了荧光光谱）.pdf

An Introduction to Fluorescence Spectroscopy User Assistance PerkinElmer Ltd Post Office Lane Beaconsfield Buckinghamshire HP9 1QA Printed in the United Kingdom. Notices The information contained in this document is subject to change without notice. PerkinElmer makes no warranty of any kind with regard to the material, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose. PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing, performance or use of this material. Copyright Information This document contains proprietary information that is protected by copyright. All rights are reserved. No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior, written permission of PerkinElmer, Inc. Copyright © 2000 PerkinElmer, Inc. Trademarks Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are protected by law. PerkinElmer is a registered trademark of PerkinElmer, Inc. 2 An Introduction to Fluorescence Spectroscopy Table of Contents Table of Contents 3 Luminescence and the nature of light 5 Fluorescence 7 Phosphorescence 11 Light scattering 14 Rayleigh-Tyndall scattering 14 Raman scattering 14 Quantitation 16 Sensitivity 18 Instrumen