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Immobilization Principles – Selecting the Surface(选择表面固定原则)
Immobilization Principles –
Selecting the Surface
ELISA Technical Bulletin - No. 1
Life
Sciences
Judy Gibbs ELISA
Corning Incorporated
Life Sciences
Kennebunk, Maine
Immuno Sorbent
Table of Contents:
Antigen/Antibody Surface
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Comple
Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Figure 1.
Structure of Polystyrene. . . . . . . . . . . . . . . . . . . 2 Introduction
Immobilization Mechanisms . . . . . . . . . . . . . . . 2 An Enzyme Linked Immunosorbent Assay (ELISA
has two major components (see Figure 1). The
Chemical Properties (Surface Flavors ). . . . . . . . 3 first is the immunological reaction that occurs
between an antigen and antibody. This reaction
is crucial and must be optimized in order for the
Physical Properties . . . . . . . . . . . . . . . . . . . . . . 5
assay to be reliable. The second component is the
surface to which antigens and antibodies are immo-
Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
bilized. The
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