Liposome Encapsulation of Fluorescent （脂质体封装的荧光）.pdfVIP

Liposome Encapsulation of Fluorescent Nanoparticles: Quantum Dots and Silica Nanoparticles * ** * Chien-Sheng Chen , Jie Yao and Richard A. Durst * Department of Food Science Technology, Cornell University Geneva, New York 14456, USA, cc328@ ** Department of Applied Engineering Physics, Cornell University Ithaca, New York 14853, USA, jy85@ ABSTRACT Liposomes are spherical vesicles consisting of phospholipid bilayers surrounding an aqueous cavity. Quantum dots (QDs) and silica nanoparticles (SNs) are Because dye-loaded liposomes provide greatly enhanced new classes of fluorescent probes that overcome the signals and their bilayer membrane can protect the dye from limitations encountered by organic fluorophores in bioassay potentially quenching species [11], liposomes have been and biological imaging applications. We encapsulated QDs successfully used as reporter particles in bioassays [12-14]. and SNs into liposomes and separated nanoparticle-loaded Several researchers reported that encapsulation of solid liposomes from unencapsulated nanoparticles by size particles into liposomes enhanced the overall mechanical exclusion chromatography. Fluorescence correlation strength of the liposome structure [11,15,16]. Therefore, spectro