冻土荒漠区分离低温适生PGPR菌的鉴定及其抗菌促生特性.PDF

30(1)94-100 中国生物防治学报 Chinese Journal of Biological Control 2014 年 2 月 冻土荒漠区分离低温适生PGPR菌的鉴定及其抗菌促生特性 * 谢永丽 ，马莉贞，徐志伟，张 英，李希来 （青海大学农牧学院/高原草地资源与生态省部共建重点实验室，西宁 810016 ） 摘要：对分离自青海昆仑山口冻土荒漠区植被根围的7 株可在4 及 10 ℃低温条件下正常生长的低温适生 菌进行鉴定分析，并检测其拮抗病原菌活性及催芽促生特性。综合理化测定、BOX-PCR 及 ERIC-PCR 指 纹图谱分析、16S rDNA 及基因gyrB 序列鉴定结果，其中6 株菌株为简单芽孢杆菌Bacillus simplex ，1 株 菌株为B. malacitensis 。平板对峙试验表明，7 株菌株对油菜菌核病原菌Sclerotinia sclerotiorum 及水稻白叶 枯病原菌 Xanthomonas oryzae pv. oryzae 具有显著拮抗效果。以菌株 KLD2 （B. simplex ）及KLD5 （B. malacitensis ）发酵菌液处理玉米种子及拟南芥幼苗，结果表明菌株发酵液可明显促进种子萌发及幼苗生长， 其鲜重、根长、须根数等表征均有显著增加。几株低温适生PGPR （plant growth promoting rhizobacteria ） 芽孢杆菌具备抗菌、催芽及促生特性，具有农业应用潜力。 关 键 词：冻土荒漠区；低温适生PGPR 菌；芽孢杆菌；分子鉴定 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2014)01-0094-07 Identification of Low-temperature Adapting PGPR Strains Isolated from Frozen Desert Area and Their Antimicrobial and Growth-promoting Activity * XIE Yongli , MA Lizhen, XU Zhiwei, ZHANG Ying, LI Xilai (Key Laboratory of Altiplaino Grassland Resource and Ecology/College of Agriculture and Animal Husbandry, Qinghai University, Xi’ning 810016, China) Abstract: Seven low-temperature adapting Bacillus strains growing under 4-10 ℃ were isolated from plant rhizospher soil in frozen desert of Kunlun Mountain. Identification via biochemistry, BOX-PCR, ERIC-PCR fingerprint, 16S rDNA sequence and gyrB sequence showed that six strains were B. simplex, while the other one was B. malacitensis. Tests on antimicrobial and growth-promoting activities indicated that they presented obvious antagonism against both Sclerotinia sclerotiorum and Xanthomonas oryzae pv. Oryzae, tw