响应曲面法优化醇提荷叶复方物超临界萃取残渣中黄酮的工艺.PDFVIP

26 2010, Vol. 31, No. 18 食品科学 ※工艺技术 甘露菁，邓泽元 * (南昌大学 食品科学与技术国家重点实验室，高等研究院，江西 南昌 330047) 对荷叶复方物超临界萃取后的残渣中黄酮进行醇提，用 Box-Behnken 响应曲面设计法，以料液比、乙醇 体积分数、温度及时间为自变量，以总黄酮得率为响应指标，用回归方程的方差分析检验模型的准确性。修正后 最优化的条件为残渣 10g 时，料液比 1:20(g/mL) 、乙醇体积分数50% 、温度80 ℃、时间80min 。在此条件下总黄 酮得率为 15.97mg/g，提取3 次结果与理论预测值相近。因此，基于Box-Behnken 响应曲面法所得的优化提取工艺 参数准确可靠，具有实用价值，可用于荷叶复方降脂产品的开发。 响应曲面法；黄酮；残渣 Optimization of Ethanol Extraction of Total Flavonoids from the Leftover Residue of a Chinese Herbal Medicine Combination after Supercritical Carbon Dioxide Extraction Using Response Surface Methodology GAN Lu-jing ，DENG Ze-yuan* (Institute for Advanced Study, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China) Abstract ：Ethanol extraction was employed to extract total flavonoids from the leftover residue of a Chinese herbal medicine combination consisting of lotus leaves, cassia seeds, hawthorn fruit and oriental water plantain rhizome after supercritical carbon dioxide extraction. The optimal conditions for the extraction of total flavonoids were explored by response surface methodology. Box-Behnken design was applied to provide experimental data for establishing a mathematical regression model describing the relationship between the extraction rate of total flavonoids and four independent variables including material-to-liquid ratio, ethanol concentration, extraction temperature and extraction time. Correlation analysis of the established model was performed, and the results indicated that it could be employed to optimize the ethanol extraction of total flavonoids from the start material.