零排放猪场基质垫层微生物群落脂肪酸生物标记多样性分析-yzxzcom.DOC

脂肪酸生物标记方法研究零排放猪舍基质垫层微生物群落多样性 刘波1(，郑雪芳1，朱昌雄2，林营志1，蓝江林1，林斌3，叶耀辉4 （1.福建省农业科学院农业生物资源研究所微生物研究中心；2.中国农业科学院环境与可持续发展研究所；3。福建省农业科学院农业工程研究所；4.福建省宁德地区农科所） 摘要：用微生物群落脂肪酸生物标记总量，分析零排放猪舍基质垫层微生物群落的数量变化，日本洛东微生物菌种处理组和零排放I号菌种处理组各层微生物脂肪酸生物标记含量变化趋势相近。基质垫层微生物群落脂肪酸生物标记检测出37个生物标记，构成微生物群落的指纹图谱，含量最高的前四个生物标记为：细菌16:00含量为431260，细菌18:1 ω9c含量为413075，厌氧细菌18:1 ω7c含量为101368，耗氧细菌i15:0含量为90328. 不同的生物标记多样性指数在基质垫层不同层次分布不同，可作为衡量特定微生物生物标记功能的一个指标。通过基质垫层微生物脂肪酸生物标记特征指数B的分析，提出了微生物群落分布的特征指标，生物标记特征指数B越高，表明微生物群落中的细菌和真菌含量越高，有利于基质垫层分解粪便排泄物，可作为基质垫层微生物群落变化优劣的特征性指标；通过基质垫层耗氧细菌和厌氧细菌脂肪酸生物标记含量比值，构建发酵指数F，作为基质垫层发酵特性的指标，发酵指数F越高，表明耗氧细菌起的作用越大，反之，耗氧细菌起的作用越小，生物标记的发酵指数可以作为研究粪便排泄物的微生物分解过程的指数。 关键词：零排放猪舍；基质垫层；磷脂脂肪酸（PLFAs）The diversity of PLFAs biomarkers for the microbial community in the stroma cushion of non-pollution pigsty LIU Bo1*, ZHENG Xue-Fang1, LIN Ying-Zhi1, LAN Jiang-Lin1, LIN Bin2, YE Yao-Hui3, LUO Yang-Fen3 (1.Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences; 2. Agricultural Engineering Research Institute, Fujian Academy of Agricultural Sciences; 3. Fujian Ningde Agricultural Research Institute) Abstract: The PLFAs biomarkers were introduced into analyzing the The diversity of PLFAs biomarkers for the microbial community in the stroma cushion of non-pollution pigsty. The microbial strains of LUODON and LINGPAIFANG #1 were used to treat the stroma cushion in the non-pollution pigsty. The results showed that the efficiency of fomentation on the pig effluent by the two strains were similar. 37 of PLFAs biomarkers were detected out by the MIDI microbial identify system, which constructed the finger printing of the microbial community in the stroma cushion in the non-pollution pigsty. The four most high of PLFAs were the bacteriun biomarker of 16:00 with 431260，bacterium biomarker of 18:1 ω9c with 413075，the anaerobic bacterium biomarker of 18:1 ω7c with 101368，the aerobic bacterium of i15:0 with 90328. The different PLFAs biomarkers were distributed in the different layers of stroma