慢性超低频复合生理频率电刺激对慢性低压缺氧兔颏舌肌肌球蛋白重 .doc

LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响 刘 熙，刘 刚，张 妮，欧 娜，张 鹏 （400038 重庆，第三军医大学西南医院呼吸内科） [摘要]目的 研究超低频复合生理频率慢性电刺激(lower physiological frequency chronic electrical stimulation,LPCES)对慢性低压缺氧兔颏舌肌肌球蛋白重链（myosin heavy chain，MHC）亚型表达和肌浆网（sarcoplasmic reticulum，SR）Ca2+摄取-释放动力学的影响。方法 成年日本大白兔24只，雌雄不限，体质量（2.15±0.10）kg，按随机数字表法分为4组（n=6）：A：正常对照组，B：慢性低压缺氧组，C：2.5 Hz电刺激组，D：(2.5+40)Hz电刺激组。B、C、D组兔在构建慢性低压缺氧模型后，给予C、D 2组慢性电刺激，刺激频率分别为超低频2.5 Hz和超低频复合生理频率(2.5+40)Hz。采用Western blot分析4组兔颏舌肌MHC亚型表达变化，采用荧光光度测定法测定SR Ca2+摄取10.86±1.26)% vs (18.61±3.19)%，P＜0.05]、SR Ca2+摄取-释放速率明显下降、摄取-释放量也明显减少（P＜0.05）。②同慢性低压缺氧组比较，慢性电刺激后各组兔MHCⅡa和MHCⅠ亚型比例明显增加（P＜0.05）、MHCⅡb亚型比例明显减少（P＜0.05），（2.5+40）Hz电刺激组较2.5Hz电刺激组显著（P＜0.05）；SR Ca2+摄取-释放速率加快、摄取-释放量增加（P＜0.05）,（2.5+40）Hz电刺激组较2.5 Hz电刺激组明显（P＜0.05）。同2.5 Hz电刺激组比较，(2.5+40)Hz电刺激组MHCⅡa和MHCⅠ亚型比例差异无统计学意义（P＞0.05）。结论 慢性电刺激后慢性低压缺氧兔颏舌肌MHC呈现Ⅱb型向Ⅱa型和Ⅰ型的转变，SR Ca2+摄取Effect of electrical stimulus at a lower physiological frequency on expressions of myosin heavy chain isoforms and kinetics of sarcoplasmic reticulum Ca2+ uptake-release in genioglossus of rabbits with chronic hypoxia Liu Xi，Liu Gang ，Zhang Ni，Ou Na，Zhang Peng (Department of Respiratory Diseases，Southwest Hospital，Third Military Medical University，Chongqing，400038，China) [Abstract] Objective To study the effect of chronic electrical stimulus at a lower physiological frequency on expressions of myosin heavy chain(MHC) isoforms and kinetics of sarcoplasmic reticulum(SR) Ca2+ uptake-release in genioglossus of rabbits with chronic hypoxia. Methods Twenty-four adult rabbits,weighing (2.15±0.10)kg,were randomized into control group(A),chronic hypoxia group(B),2.5Hz electrical stimulus group(C) and (2.5+40)Hz electrical stimulus group(D).Rabbits in group C and D received electrical stimulus on genioglossus at a frequency of 2.5Hz and (2.5+40)Hz respectively,while those in group B received no electrical stimulus,after they had been fed with free access to food and water in a hypoxia cabin 10 h a day for 4 weeks.Exprseeions of M