慢性阻塞性肺疾病及糖尿病大鼠肺组织β2肾上腺 - 第三军医大学学报.doc

慢性阻塞性肺疾病及糖尿病大鼠肺组织β2肾上腺素能受体表达水平的研究 翟聪颖1 ，杨艳平1，李 哲2，贾平东1，王 岩3（济南 250013 山东大学附属济南市中心医院：呼吸内科1，病理科2，淄博 255100 淄博市第五人民医院神经内科3） 提要：目的 观察慢性阻塞性肺疾病大鼠、糖尿病大鼠及慢性阻塞性肺疾病复合糖尿病大鼠肺组织β2肾上腺素能受体mRNA表达的变化。方法 60只雄性5周龄Wistar大鼠随机分为慢性阻塞性肺疾病组（COPD组，n=14）、糖尿病组（DM组，n=16）、慢性阻塞性肺疾病合并糖尿病组（COPD+DM组，n=18），正常对照组（C组，n=12）。采用实时荧光定量PCR的方法检测肺组织β2肾上腺素能受体mRNA的表达，并进行比较。结果 COPD组、DM组、COPD+DM组大鼠肺组织的β2肾上腺素能受体mRNA的表达量显著低于正常对照组（P＜0.01)，并且COPD+DM组的基因表达量显著低于COPD组和DM组（P＜0.01)。COPD组与DM组之间无差异（P＞0.05)。结论 慢性阻塞性肺疾病、糖尿病可使大鼠肺组织的β2肾上腺素能受体mRNA的表达明显降低，而慢性阻塞性肺疾病合并糖尿病时其降低更加显著。 关键词: 荧光实时定量PCR；慢性阻塞性肺疾病；糖尿病；β2肾上腺素能受体 中图分类号：R563.3 R587.1 Detection of β2-AR mRNA Expression in rat lung tissue of Chronic Obstructive Pulmonary Disease and Diabetes Mellitus ZHAI Cong-ying1，YANG Yan-ping1，LI Zhe2，JIA Ping-dong1，WANG Yan3 (Jinan Central Hospital Affiliated to Shandong University : 1.Department of Respiratory Medicine， 2.Pathology Department， Jinan，250013;3.Neurology Department， The Fifth Hospital of Zibo， Zibo，255100) Abstract: Objective To investigate the expression of β2-AR mRNA in rat lung tissue of Chronic Obstructive Pulmonary Disease， Diabetes Mellitus and Chronic Obstructive Pulmonary Disease with Diabetes. Methods We divided 60 Rats（5-week-old） into four groups， the COPD group， the DM group， the COPD with DM group and the C group randomly. Then we used Real-time Quantitative PCR to examine the espression of β2-AR mRNA. Results The expression of β2-AR mRNA in the three model groups was much lower than that in the C group( P＜0.01).And its expression in the COPD with DM group is much lower than that in the COPD group and in the DM group( P＜0.01). But there was no difference between the DM group and the COPD group（P＞0.05). Conclusion Both COPD and DM can lower the expression of β2 -AR mRNA in lung tissue obviously， and COPD with DM can lower it more obviously. Key words: real-time polymerase chain reaction(PCR)；Pulmonary Disease， Chronic Obstructive Diabetes Mellitus， Receptors， Adrenergic， beta-2 β肾上腺素能受体（β-AR）在肺内有很高的