注射用苦参碱对阿霉素致大鼠心脏重构的影响.pdf

Drug Evaluation Research 第 39 卷 第 2 期 2016 年 4 月 • 193 • 注射用苦参碱对阿霉素致大鼠心脏重构的影响 1 2 2 2 2* 王燕琴 ，初而复 ，李 欣 ，张明亮 ，卢均坤 1. 佳木斯大学校医院，黑龙江 佳木斯 154003 2. 佳木斯大学附属第一医院，黑龙江 佳木斯 154003 摘 要：目的 研究注射用苦参碱对阿霉素引起大鼠心脏重构的影响。方法 将 52 只大鼠随机分为：对照组、模型组、苦 参碱（100 mg/kg）组，尾 iv 阿霉素制备心肌损伤模型，采用超声心动图检测大鼠心脏左心室舒张末期内径（LVEDd ）、左 室射血分数（LVEF ）和缩短分数（FS ）；生理记录仪检测大鼠左心室舒张末期压（LVEPD ）、收缩压（LVPS ）、室内压最大 收缩/舒张速率（±dp /dtmax ）；称大鼠体质量，脊髓离断法处死大鼠，称大鼠心脏质量并计算心脏指数；试剂盒法检测大鼠心 脏组织中谷胱甘肽过氧化物酶（GSH-Px ）、超氧化物歧化酶（SOD ）活性以及丙二醛（MDA ）水平；应用流式细胞术检测 大鼠心肌细胞凋亡水平。结果 与模型组比较，苦参碱组大鼠 LVEDd 值显著降低、EF 和 FS 值显著升高；LVEPD 显著降低， LVPS 、dp /dtmax 及－dp /dtmax 均显著升高；心脏指数、心肌细胞凋亡率显著降低；心肌细胞内 GSH-Px、SOD 活性明显升高， MDA 水平显著降低。结论 注射用苦参碱减轻阿霉素引起的心肌细胞凋亡，改善心脏重构及心脏功能，可能与改善心肌细 胞氧化应激水平有关。 关键词：苦参碱；阿霉素；心脏重构；凋亡；氧化应激 中图分类号：R965 文献标志码：A 文章编号：1674-6376 (2016) 02-0193-05 DOI: 10.7501/j.issn.1674-6376.2016.02.007 Effects of matrine on cardiac remodeling in rats induced by Adriamycin 1 2 2 2 2 WANG Yan-qin , CHU Er-fu , LI Xin , ZHANG Ming-liang , LU Jun-kun 1. Hospital of Jiamusi University, Jiamusi 154003, China 2. 1st Affiliated Hospital of Jiamusi University, Jiamusi 154003, China Abstract: Objective To study the regulation of matrine on adriamycin-induced cardiac remodeling in rats. Methods Sixty SD rats were randomly divided into control group, model group, and matrine group. The cardiac LVEDd, LVEF, and FS values of three groups were detected by echocardiography. The values of LVEPD, LVPS, ±dp /dtmax were detected by physiological recorder. The weights of rats were measured. The rats were killed by the method of spinal cord transection, the weight of the heart of rat was measured and the heart index was calculated. The levels of oxidative stress were evaluated by using reagent kit of glutathione perox