白细胞介素-4对肺泡上皮细胞钠离子通道β亚基 - 第三军医大学学报.doc

白细胞介素-4对肺泡上皮细胞钠离子通道β亚基调控研究 张 维，王导新 （重庆医科大学附属第二医院呼吸内科，重庆 400010） 摘 要： 目的 研究不同时间不同浓度白细胞介素-4（IL-4）作用下A549细胞钠离子通道β亚基（ENaC-β）的变化情况。方法 以A549细胞为模型，在含IL-4培养基不同时间不同浓度培养下，分别用免疫印迹法以及RT-PCR法测定ENaC-β蛋白表达以及mRNA变化转录情况。结果 IL-4可以呈浓度、时间依赖性的下调ENaC-β的表达，这种蛋白表达的下调作用是通过基因调控起作用的。IL-4(1ng/ml)培养A549细胞3h， ENaC-β蛋白及mRN开始下降，在6h后出现显著差异（P0.05），不同浓度的IL-4（0.1、10、100ng/ml）Interleukin-4 Decreases Expression of the Epithelial Sodium Channel βENaC in A549 Cells ZHANG Wei ,WANG Dao-xin （The Respiratory Department of Second Affiliated Hospital of Chongqing Medical University, ChongQing 400016） Abstract： Objective To observe the effects of IL-4 on the expression of Epithelial Sodium Channel βENaC. Methods A549 cells were cultured with different concentrations or with different time of IL-4. Then ENaC-β protein and mRNA were detected by western-blot and RT-PCR. Results IL-4 can decreased the expression of Enac-βin A549 cells in a time- and dose-dependent manner, which was via the way of gene transformation. When A549 cells were cultured with IL-4 at the concentration of 1ng/ml for 3, 6, 12, 24, 48 hours the expression ofβ-ENaC and mRNA were decreased with a time dependce manner. Statistical analysis shows at 6 hours it shows a significant difference (p0.05). When cells were cultured with different concentration of 0, 0.1, 1, 10ng/ml for 12 hours the β-ENaC expression and mRNA show a dose dependence manner. Statistical analysis shows at 0.1ng/ml it shows a significant difference. Conclusion IL-4 was always thinked as a anti-inflammatory factor and have a protection in ALI/ARDS. But in this experiment it seems have a different role in the disease ,IL-4 can decreases the expression of ENac-β, also can down-regulate the active alveolar fluid clearance ,which may make the outcome worse. Key words：IL-4；ENaC-β；Acute lung injury / Acute respiratory distress syndrome 富含蛋白质的肺泡水肿液聚集是急性肺损伤(acute lung injury，ALI)和急性呼吸窘迫综合征（acute respiratory distress syndrome，ARDS）的共同的病理特征。是否能有效清除肺泡内过多液体，维持肺泡腔内相对干燥的环境，对于有效的气体