雌性大鼠中段尿道结构的年龄变化.doc

雌性大鼠尿道结构的龄变化 胡丽娟杜广辉 摘要目的观察尿道括约肌各组织成分年龄变化方法取6、12、24、32月龄雌性未生育Fisher大鼠尿道常规石蜡包埋切片，Masson’s Trichrome 染色，光学显微镜观察定量分析尿道括约肌组织和空间形态的变化结果观察发现尿道横纹肌含量从12月龄开始减少从24月龄开始尿道粘膜变薄、粘膜下血管数量减少、尿道嵴面积缩小、尿道腔最长径/最短径比值增大，胶原结缔组织含量增加；尿道背侧壁厚度变薄，尿道腔面积扩大见于32月龄组。尿道平滑肌和尿道总面积随年龄增加没有发生明显变化。 结论随着年龄增长雌性大鼠尿道，尿道功能障碍性疾病的形态学基础。关键词雌性大鼠Age Effects on the Morphometry of Female Rat Urinary Sphincter HU Li-juan, DU Guang-hui, HU Wei-feng, CHEN Biao, ZHANG Hui-ping, YE Zhang-un. （Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China） Abstract：Objective To study the aging changes of histological components of the urinary sphincter in female rats and investigate the morphological aspects for understanding the mechanism of age related urinary continence dysfunction. Methods Twenty-eight adult female nonporous Fisher rats of different age were divided averagely into four groups: group I: 6 months, group II: 12 months, group III: 24 months, group IV: 32 months. The dorsal urethra wall thickness, urethral lumen area, cross section area of crista urethralis, Mucosa thickness and area, number of submucosal vessels, the mount of collagen and striated and smooth muscle fibers of the mid-urethra cross-sections of adult female rats in different age groups were evaluated by computerized morphometric analysis system after staining with Masson’s Trichrome procedure. Results It was noted that the amount of striated muscle fibers decreased remarkably from 12 months old female rats( P12/6=0.031). The mucosal area, the number of submucosal vessels, the cross section area of crista urethralis decreased from 24 months (each P0.05). While the amount of collagen (P=0.047) and the radio of the longest and shortest urethral diameters(P=0.049) increased . The urethral lumen area (P=0.026) and the dorsal urethra wall thickness (P=0.034) changed in 32 months rats. It was verified that the whole urethra area and the amount of smooth muscle fibers did