不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点.PDFVIP

比较不同品系小鼠间皮肤移植致敏后抗供体特异性抗体（DSA ）的变化特点。 将小鼠 分为 Balb/c → C57BL/6 皮肤移植组（6 对）和 Balb/c → C3H 皮肤移植组（6 对）。皮肤移植后收集 d0、d2、 d4、d7、d13、d17、d28、d35、d42、d49、d56 时间点血清并检测 DSA-IgG 和 DSA-IgM 。小鼠皮肤移植致 敏后 1 周内 DSA-IgG 水平升高不明显， 1 ～4 周内 IgG 水平持续快速增长，4 ～8 周 IgG 水平处于高峰平台稳定。 皮肤移植后 8 周内 DSA-IgM 无明显变化。Balb/c → C57BL/6 皮肤移植组 DSA-IgG 水平明显低于 Balb/c → C3H 组， 两组在 d2、d17、d28、d35、d42、d49 和 d56 时间点的 IgG 水平差异有统计学意义（均为 P0.05 ），两组DSA- P IgM 在各时间点比较差异无统计学意义（均为 0.05 ）。 利用 Balb/c → C3H 适当提前皮肤移植后的肾移 植手术时间，或改用免疫反应性更弱的 Balb/c → C57BL/6 组合有望建立排斥反应更弱的移植肾 AMR 模型。 　皮肤移植；供体特异性抗体（DSA ）；抗体介导的排斥反应（AMR ）；小鼠；免疫反应；肾移植； 动物模型；免疫球蛋白（Ig ） R617 ，R392.4　 A　 1674-7445 （2017 ）03-0006-04 Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation Liao Tao, Zhao Daqiang, Li Siwen, Han Fei, Luo Zihuan, Liu Xiaonan, Sun Qiquan. Department of Renal Transplantation, the Third Affi liated Hospital of Sun Yat-sen University, Guangzhou 510760, China Corresponding author: Sun Qiquan, Email: sunqiq@mail.sysu.edu.cn Abstract Objective 【 】 To compare the change features of anti-donor specifi c antibody (DSA) in different species of sentitized mice after skin transplantation. Methods All mice were divided into the Balb/c → C57BL/6 (6 pairs) and Balb/c → C3H skin transplantation groups (6 pairs). At d0, d2, d4, d7, d13, d17, d28, d35, d42, d49 and d56 after skin transplantation, the serum sample was prepared for detection of DSA-IgG and DSA-IgM levels. Results Moderate increase was noted in the DSA-IgG level in the sensitized mice within 1 week after skin transplantation. The IgG level was signifi cantly increased within 1-4 week and peaked and stabilized within 4-8 week. No signifi cant variation was observed in the DSA-IgM level