三种常见阴道分泌物念珠菌检测手段方法学评价.doc

三种常见阴道分泌物念珠菌检测手段方法学评价[摘要] 目的 评价三种白带念珠菌检测手段的方法学差异。方法 对540例拟诊为念珠菌阴道炎患者的分泌物同时做盐水涂片、快速染色镜检和真菌培养。结果 盐水涂片法念珠菌阳性率为60.4%，染色法和培养法阳性率分别为75.8%、78.9%。盐水涂片法操作简单、快捷、阳性率较低，与染色法和培养法阳性率差异有统计学意义（P＜0.01）；后两者阳性率较高，差异无统计学意义（P＞0.05）。结论 相比较，培养法能做鉴定和药敏，能指导临床用药，缺点是需要特殊仪器和试剂、操作繁琐、耗时长，不利于念珠菌阴道炎的早期诊断和治疗；染色镜检法操作简单、快速，有利于早期诊断，但不能指导临床用药。 [关键词] 阴道分泌物；念珠菌；阴道炎 [中图分类号] R711.3 [文献标识码] B [文章编号] 1673-9701（2011）26-89-02 Appraisal of Three Common Means of Examination of Common Vagina Secretion Monilia WANG Hong XU Meng Clinical Laboratory of First People’s Hospital of Xinyang City in Henan Province， Xinyang 464000，China [Abstract] Objective To evaluated three kinds of vaginal candidiasis methodological differences in testing methods. Methods All 540 patient’s clinically diagnosed as of candida vaginitis discharge while doing salt water smear，the microscope after rapid dyeing and the fungus culture. Results Positive rate of Monilia was 60.4% demonstrated by the salt water smear；75.8% positive rate by the method of rapid dyeing microscope，78.9% positive rate by the method of fungus culture. There was significant difference in the positive rate between salt water smear，the microscope after rapid dyeing and the fungus culture（P＜0.01）；The positive rate of the microscope after rapid dyeing and the fungus culture were higher，but there were no significant difference between them（P＞0.05）. Conclusion In Comparison，the identification of Monilia and medicine sensitive examination can be effectively demonstrated by fungus culture method，which is helpful to instruct the clinical medication but with several shortcomings like special instrument and reagent are indispensable；complex operation；long time-consuming，these impede early diagnosis of the vagina secretion Monilia and treatment. Relative Simplicity of operation enables rapid dyeing microscopic exam method to obtain early diagnosis，but untenable instruct the clinical medication. [Key words] Vaginal secretion；Monilia；Vaginitis 念珠菌阴道炎是临床常见的妇科疾