乌贼墨对小鼠T细胞亚群及T细胞活化影响.doc

乌贼墨对小鼠T细胞亚群及T细胞活化影响[摘要] 目的：研究乌贼墨对小鼠T细胞亚群和T细胞活化的影响。方法：实验组小鼠每天灌胃5%的乌贼墨0.5 ml，连续7 d。对照组小鼠给予等量生理盐水。流式细胞术检测小鼠外周血CD4+T细胞百分比、CD4+/CD8+比值及IL-2αR（CD25）表达。结果：实验组小鼠外周血CD4+T细胞百分比、CD4+/CD8+比值及IL-2αR（CD25）表达较对照组小鼠均有明显升高，而CD8+T细胞却无显著性变化。结论：乌贼墨通过增高CD4+T细胞百分比、CD4+/CD8+比值及IL-2αR（CD25）表达对小鼠免疫功能进行调节。 [关键词] 乌贼墨；免疫作用；T细胞亚群；T细胞活化 [中图分类号] R285.5 [文献标识码]A [文章编号]1673-7210（2008）07（c）-025-02 Research on the effect of the Squid Ink on T-lymphocyte subsets and the activation of T cell of KM mice WANG Xin1，2, LI Guo-ming1，2 (1.Department of Clinical Micro-Organism Ecsomatics, Ecsomatics School, Guangdong Medical College,Dongguan 523808,China ; 2.Department of Microbiology and Immunology, Zhanjiang 524023, China) [Abstract] Objective: To study the effects of the squid ink on T-lymphocyte subsets and the activation of the squid ink in KM mice.Methods: 5% squid ink was given to the experimental group mice ig with 5 ml.Blank control group was set up with NS.After administration of 7 consecutive days, the percentage of CD4+ T cell, CD4+/CD8+ and the quantity of IL-2αR(CD25) in KM mouse blood were measured by flow cytometry(FCM). Results:The percentage of CD4+ T cell, CD4+/CD8+ and the quantity of IL-2αR(CD25) in KM mouse blood of experimental group(EG) was higher than control group(CG) ， but the percentage of CD8+ T cell had no significant change.Conclusion: The squid ink promotes immune function of KM mouse by the rising of the percentage of CD4+ T cell , CD4+/CD8+ and the quantity of IL-2αR(CD25). [Key words] Squid ink; Immunifaction; T-lymphocyte subsets; Activation of T cell 乌贼墨由乌贼墨囊中的分泌腺合成，在墨囊中储存，是乌贼遇到天敌时喷出来染黑周围水域的黑色物质。我国医药对乌贼的应用历史悠久，对乌贼墨的药用始于止血。现代对乌贼墨的研究是从乌贼墨中黑色素的结构及其生物合成开始的。20世纪90年代初日本研究人员通过动物实验证明乌贼墨可明显抑制甚至消除大鼠的移植纤维恶性肿瘤，同时认为这不是因为乌贼墨直接作用于癌细胞，而是通过提高免疫力而杀死癌细胞[1]。我国的研究人员也证明了乌贼墨可通过刺激小鼠分泌多种细胞因子抗肿瘤[2-4]。本研究主要从乌贼墨对小鼠T细胞亚群和T细胞活化的影响方面，研究乌贼墨对免疫功能的影响，为进一步应用乌贼墨提供实验和理论依据。 1材料与方法 1.1实验试剂及仪器 FITC anti-mouse CD3ε、PE anti-mouse CD4、PE anti-mouse CD8α、FITC anti-mouse CD25（美国BioLeg